Yafteh

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Menopause and decrease of estrogenic hormones lead to osteoporosis. Treatment of osteoporosis by estrogenic hormones like estrogen in women has resulted in secondary complications such as cancers of breast and genital system. Activity of osteoclasts for removing bone is necessary to secretion of H+ ions on bone matrix. Today, using proton pump inhibitors to inhibit the activity of osteoclasts is under study. In other hand, recent researches show that these inhibitors by decrease absorbing of calcium intestine, can increase the risk of fracture. According to physiology of osteoclasts and the vitamin D mechanism on absorbing calcium from intestine, the combined effect of vitamin D3 and omeprazole were studied. Materials and Methods: 64 female Sprague dawlley rats (160 to 180 gr ) were selected and divided into 8 groups ( 8 in each ): 1- Sham group, without ovariectomy, 2- ovariectomized group without treatment, 3- ovariectomized group, treated with vitamin D3 100 µg ∕ kg, 4- ovariectomized group, , treated with vitamin D3 400 µg ∕ kg, 5- ovariectomized group, treated with omeprazole 20 mg/ kg and vitamin D3 100 µg/kg, 6- ovariectomized group, treated with omeprazole 20 mg/ kg and vitamin D3 400 µg/kg, 7- ovariectomized group, treated with omeprazole 20 mg/ kg, 8- ovariectomized group, treated with DMSO as a solvent. A week after ovariectomy, treatment of 3th to 8th groups began and continued for 12 weeks. During the sampling, under general anesthesia, blood were sampled and serum was prepared, and then lumbar vertebrae, femur bones and the right tibia separated and were put in the fixative solution. Serum alkaline phosphatase and Ca were measured. Resistance of femur bones were measured against fracture. Histological section prepared from L3 vertebrae and the end of tibia bones. The volume density of trabeculae of the L3 vertebrae body of samples was measured by stereologic rules. The number of osteoclasts in tibias sections was measured morphometrically, and the density of L4 vertebrae was determined based on Archimedes principle . The mean of variables was compared by SPSS 12 software. To compare the mean of alkaline phosphotase, serum Ca, the density of L4 vertebrae and the resistance of femur bones against fracture, we used ANOVA and Games-Howells test, and to compare the mean of volume density of trabeculae and the number of osteoclasts, Mann- Whitney test in p<0. 05 was used. Results: After 12 weeks, ovariectomy made severe osteoporosis in the 2nd and 7th groups . In the 1st, 3rh, 4th, 6th and 8th, no osteoporosis was delected, but in the 5th group, osteoporosis was detected slightly. Conclusion: Vitamin D treatment and almost DMSO prevent osteoporosis progressing. omeprazole treatment alone can prevent osteoporosis but in combination with a suitable dose of vitamin D, it can prevent osteoporosis and its progress.

Keywords: : Menopause, Osteoporosis, Vitamin D3, Omeprazole, Ovariectomy, DMSO, Bone density.

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