Background : Salmonella enterica serotype Enteritidis is one of the most important cause of non-typhoidal salmonellosis, a clinically less severe infection than typhoid fever. The present study aimed to detection of virulence genes in the S. enteritidis isolated from the food samples by multiplex-PCR method.
Materials and Methods: In this descriptive cross-sectional study was performed during a year in 2014 on the 1250 no duplicative and non-repetitive food samples. M-PCR assay was done in order to detection of invA ،ttrC، mgtC، spi4D and agfA genes.
Results: Totally, sixty S. enteritidis strains were obtained from the poultry meat (38 strains, 61.6%) and the eggs (23 strains, 38.3%), respectively. The highest and lowest prevalence of these genes were related to mgtC (51.6%) and spi4D (1.3%).
Conclusion: Evaluation of virulence genes in the S. Enteritidis isolated from food samples are useful because of the incidence of the virulence elements and efficiency of M-PCR in the epidemiological study and evaluation of intraspecies genes transfer in the various samples.