Volume 18, Number 3 (12-2016)                   yafte 2016, 18(3): 88-94 | Back to browse issues page


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Mirzadeh S, Mahmoudi R, Amini Q. Evaluation of virulence genes in the Salmonella enteritidis isolated from food ‎samples by multiplex-PCR. yafte. 2016; 18 (3) :88-94
URL: http://yafte.lums.ac.ir/article-1-2363-en.html

Associate Professor
Abstract:   (891 Views)

Background : Salmonella enterica serotype Enteritidis is one of the most important cause of non-typhoidal ‎salmonellosis, a clinically less severe infection than typhoid fever. The present study aimed to ‎detection of virulence genes in the S. enteritidis isolated from the food samples by multiplex-PCR ‎method.‎

Materials and Methods: In this descriptive cross-sectional study was performed during a year in 2014 on the 1250 no ‎duplicative and non-repetitive food samples. M-PCR assay was done in order to detection of ‎invA ‎،ttrC،‎ mgtC،spi4D and agfA genes.‎

Results: Totally, sixty S. enteritidis strains were obtained from the poultry meat (38 strains, 61.6%) and ‎the eggs (23 strains, 38.3%), respectively. The highest and lowest prevalence of these genes were ‎related to mgtC (51.6%) and spi4D (1.3%).‎

Conclusion: Evaluation of virulence genes in the S. Enteritidis isolated from food samples are useful because ‎of the incidence of the virulence elements and efficiency of M-PCR in the epidemiological study ‎and evaluation of intraspecies genes transfer in the various samples.‎

Full-Text [PDF 235 kb]   (437 Downloads)    
Type of Study: Research |
Received: 2016/12/19 | Accepted: 2016/12/19 | Published: 2016/12/19

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