Volume 16, Issue 2 (9-2014)                   yafte 2014, 16(2): 99-106 | Back to browse issues page

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Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran, soleim_h@modares.ac.ir
Abstract:   (7877 Views)
Background : The aim of the present study was to identify a suitable cell line for studies of recombinant protein production in eukaryotic system. After transfection, altered expression levels of RNA and its target protein were analyzed. Materials and Methods: To investigate the in vitro expression of E6 protein of human papillomavirus type 16 in cell culture, the plasmid pcDNA3-E6, and two different cell lines MCF7 and CHO were used. Following transfection, RNA expression was determined by RT- PCR and its protein target was evaluated by Western blot using SDS-PAGE gel and Anti-E6 monoclonal antibody. Results: In this study due to the low concentration of proteins in MCF7cells, RT-PCR was positive and Western blotting was negative, while RT-PCR and Western blotting were positive for CHO cell lines. Conclusion: Regarding the results, the use of CHO expression system as a tool for efficient transfection of recombinant protein production is suggested.
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Type of Study: Research |
Received: 2014/09/30 | Accepted: 2014/09/30 | Published: 2014/09/30

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