Background : Salmonella enterica serotype Enteritidis is one of the most important cause of non-typhoidal ‎salmonellosis, a clinically less severe infection than typhoid fever. The present study aimed to ‎detection of virulence genes in the S. enteritidis isolated from the food samples by multiplex-PCR ‎method.‎

Materials and Methods: In this descriptive cross-sectional study was performed during a year in 2014 on the 1250 no ‎duplicative and non-repetitive food samples. M-PCR assay was done in order to detection of ‎invA ‎،ttrC،‎ mgtC،‎ spi4D and agfA genes.‎

Results: Totally, sixty S. enteritidis strains were obtained from the poultry meat (38 strains, 61.6%) and ‎the eggs (23 strains, 38.3%), respectively. The highest and lowest prevalence of these genes were ‎related to mgtC (51.6%) and spi4D (1.3%).‎

Conclusion: Evaluation of virulence genes in the S. Enteritidis isolated from food samples are useful because ‎of the incidence of the virulence elements and efficiency of M-PCR in the epidemiological study ‎and evaluation of intraspecies genes transfer in the various samples.‎