Volume 20, Issue 4 (1-2019)                   yafte 2019, 20(4): 1-7 | Back to browse issues page

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Shabani M, Sadeh M, Falsafi S, Amini K. The Prevalence of Papillomavirus-16 and -18 isolated from women with cervical cancer using Multiplex PCR. yafte. 2019; 20 (4) :1-7
URL: http://yafte.lums.ac.ir/article-1-2372-en.html
Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran.
Abstract:   (408 Views)

Background: Women infected with human papillomavirus, especially types 16 and 18, are at risk of cervical cancer. The purpose of this study was to determine the frequency of papillomavirus-16 and -18 in women with cervical cancer using multiplex-PCR.
Materials and Methods: In this experimental study, after collecting blood samples, viral DNA was extracted using a Cinaclone kit, and PCR, with specific primers, was performed to detect HPV-16 and HPV-18. PCR products were analyzed by 1% agarose gel electrophoresis.
Results: Of 60 samples, 19 were infected with HPV. The results showed that the frequency of genotype HPV-16 and HPV-18 was 8 (42.1%) and 11 (57.9%), respectively.
Conclusion: The study showed that using PCR with specific primers for the detection of HPV-16 and HPV-18 is a convenient and accurate method. The results of this study indicate the relationship between HPV and cervical cancer.
 

Full-Text [PDF 267 kb]   (101 Downloads)    
Type of Study: Research | Subject: میکروب شناسی
Received: 2017/01/6 | Accepted: 2017/05/9 | Published: 2019/02/24

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