Background : Antioxidants are compounds that protect the body against damage caused by oxidative stress that induce by free radicals. The aim of present work is to compare the various antioxidative activities of leaves hydroalcholic extract and essential oil of Vitex pseudo-negundo. Materials and Methods: In this experimental study, leaves hydroalcholic extract and essential oil of Vitex pseudo-negundo were prepared and then radical scavenging activity of samples was assessed by using Diphenylpicrylhydrazyl (DPPH). Total antioxidant capacity samples was assessed by method of phosphomolybdat. The amount of total phenol and flavonoid samples was assessed by Folin-Ciocalteu and Zhishen methods Results: The results showed that total antioxidant capacity Leaves hydroalcholic extract and essential oil of Vitex pseudo-negundo is (1.76±0.25 2.12±0.60), phenol content (133.11±3.00 22.00±2.00), and flavonoid content (18.00±1.00 5.50±4.00). In the DPPH scavenging assay, the IC50 (the concentration required to scavenge 50% of radical) values of leaves hydroalcholic extract and essential oil and Butylated hydroxytoluene (BHT) as refernce were (224.75±4.52 133.00±3 3.88±1μg/ml), respectively. Conclusion: This study showed that Vitex pseudo-negundo extract is an source easily accessible of natural antioxidants and it may be suitable for use in food and pharmaceutical applications.

Keywords: Vitex; hydroalcholic extrac, Essential oil, Total phenols; Total flavonoids, Antioxidant activity.

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