TY - JOUR T1 - The long-term effect of bilateral tubal ligation on ovary and uterus in rat (Histopathological study) TT - تأثیر دراز مدت بستن دو طرفی لوله های رحمی بر تخمدان و رحم در موش بزرگ (مطالعه هیستوپاتولوژیک) JF - Yafteh JO - Yafteh VL - 15 IS - 3 UR - http://yafte.lums.ac.ir/article-1-1365-en.html Y1 - 2013 SP - 46 EP - 53 KW - Bilateral tubal ligation KW - Side effects KW - Ovarian follicles KW - Endometrium KW - Rat. N2 - Background : The aim of this study was to evaluate ovarian follicular numbers and uterus changes at 3, 6 and 9 months after bilateral tubal ligation (BTL) in rat. Materials and Methods: In this experimental study 60 female rats (Sprague-Dawley) , aged two months were divided into control and BTL groups (30 per group). Uterine tubes ligation was done bilaterally similar to pomeroy method. Each 3 months, 10 control and 10 BTL animals were selected randomly and then left ovary and uterus were sampled and fixed in formal saline solution. Paraffin sections (5-µ thickness) prepared and were stained by H and E and trichrom methods. Endometrial changes were studied qualitatively and the numbers of follicle types were counted. Data were analyzed by Mann-Withney U test with SPSS 13 software (p M3 ER -