per
Lorestan University of Medical Sciences
Yafteh
1563-0773
2981-0779
2012-07
14
2
5
13
article
Antioxidant properties of leaves essential oil and hydroalcoholic extract Vitex pseudo-negundo
hasan ahmadvand
hassan_a46@yahoo.com
1
hamze amiri
2
saeede Ekbatan Hamadani
3
shahrokh Bagheri
4
Razi Herbal Medicine Research Center
Faculty of Basic science, Lorestan University, Khoramabad, Iran
Islamic Azad University, Broujerd Branch, Broujerd, Iran
Faculty of Medicine, Lorestan University of Medical Sciences, Khoramabad, Iran
Background : Antioxidants are compounds that protect the body against damage caused by oxidative stress that induce by free radicals. The aim of present work is to compare the various antioxidative activities of leaves hydroalcholic extract and essential oil of Vitex pseudo-negundo.
Materials and Methods: In this experimental study, leaves hydroalcholic extract and essential oil of Vitex pseudo-negundo were prepared and then radical scavenging activity of samples was assessed by using Diphenylpicrylhydrazyl (DPPH). Total antioxidant capacity samples was assessed by method of phosphomolybdat. The amount of total phenol and flavonoid samples was assessed by Folin-Ciocalteu and Zhishen methods
Results: The results showed that total antioxidant capacity Leaves hydroalcholic extract and essential oil of Vitex pseudo-negundo is (1.76±0.25 2.12±0.60), phenol content (133.11±3.00 22.00±2.00), and flavonoid content (18.00±1.00 5.50±4.00). In the DPPH scavenging assay, the IC50 (the concentration required to scavenge 50% of radical) values of leaves hydroalcholic extract and essential oil and Butylated hydroxytoluene (BHT) as refernce were (224.75±4.52 133.00±3 3.88±1μg/ml), respectively.
Conclusion: This study showed that Vitex pseudo-negundo extract is an source easily accessible of natural antioxidants and it may be suitable for use in food and pharmaceutical applications.
http://yafte.lums.ac.ir/article-1-748-en.pdf
Vitex; hydroalcholic extrac
Essential oil
Total phenols; Total flavonoids
Antioxidant activity.
per
Lorestan University of Medical Sciences
Yafteh
1563-0773
2981-0779
2012-07
14
2
5
12
article
Nutritional assessment in hospitalized patients of orthopedic ward at hospital admission and discharge time in Shohadaye Ashayer hospital
mahnaz Mardani
msmardani@yahoo.com
1
fatemeh Seifi
2
farzad Ebrahimzadeh
3
Faculty of Health and Nutrition
Background: Malnutrition is one of the significant factors in extending hospitalization period, augmenting disease complications, enhancing treatment and hospitalization costs, and increasing deaths in hospitals. This study was conducted to investigate patients, nutritional conditions, before and discharge time in the orthopedic ward of Shohadaye Ashayer hospital in 2010.
Materials and Methods: In this cross-sectional descriptive study 283 patients, aged 18 and over, in the orthopedic ward were selected.A previously developed questionnaire containing 15 quantitative and qualitative factors was completed for subjects in two stages (at their hospital admission and their discharge time). The patients, weight and height were also measured during these two stages. The collected data were then analyzed using SPSS software.
Results: 12.7% of the patients had severe malnutrition, 7.4% had moderate malnutrition and 79.9% had mild malnutrition. The length of hospital stay was found to be an important factor in causing malnutrition. In this study significant statistical relationship was found between the length of hospital stay, sex, the recent 1-month weight loss, previous operation and the severity of malnutrition (P0.05).
Conclusion: Based on the findings of this study, it can be claimed that there is a significant statistical relationship between the length of hospital stay and the severity of malnutrition.
http://yafte.lums.ac.ir/article-1-1182-en.pdf
Malnutrition
Length of hospital stay
Orthopedic ward of Shohadaye Ashayer hospital.
per
Lorestan University of Medical Sciences
Yafteh
1563-0773
2981-0779
2012-07
14
2
15
22
article
Physical features and nutritional value of golden and red delicious apples (Malus, Domestica Borkh) grown in Lorestan, Iran
ebrahim falahi
1
esfandiar Hasani Moghadam
2
sajad Roosta
sajjad.roosta @yahoo.com
3
Razi Herbal Medicines Research Center
Razi Herbal Medicines Research Center
Background : Apples have been known as nutritious and natural medicines. The aim of this study was to determine the amounts of nutrients compounds of golden and red delicious apples produced in Lorestan, Iran.
Materials and Methods: Apple cultivars were harvested from different locations throughout Lorestan province in September 2008. The samples were dried at 80 º C for two days. The dry materials were grounded to analyse the elements. Phosphorous calcium, iron, and zinc and sodium and potassium were measured by spectrometry, atomic absorption and flame photometry, respectively. Other measured factors were energy, protein, carbohydrate, water, ash, and fiber.
Results: The amounts of water, soluble solid products, ash, and fiber in red apples varieties were 78, 17.9, 1.44, and 0.72 percent, respectively. These values for golden delicious apples were 77, 16, 1.42, and 0.72 percent, respectively. Energy, sugars, and protein contents of red delicious apples were 73 calories, 18, and 0.27 g/100, respectively. These amounts for golden varieties were 83 calories, 20.6, and 0.2 g/100, respectively. K, Ca, P, Na, Fe, and Zn contents of red delicious apples were 63.8, 27.6, 9, 4.8, 0.24, and 0.14 mg/100, respectively. These values for golden delicious variety were 66.3, 27.7, 8.8, 4.5, 0.23, and 0.14 mg/100, respectively.
Conclusion: Energy, carbohydrates, and calcium contents of apples varieties produced in Lorestan province were more than their contents in Iranian Food Composition Tables. However, their iron contents were less than it.
http://yafte.lums.ac.ir/article-1-749-en.pdf
Apples
Nutritional Values
Minerals
Carbohydrates
Lorestan
per
Lorestan University of Medical Sciences
Yafteh
1563-0773
2981-0779
2012-07
14
2
23
29
article
The effect of Satureja Khuzestanica Essential Oil (SKEO) on convulsion induced by Nicotine in male mice
rahele Assaei
asaee_ra@yahoo.com
1
masoum Bashiri
2
naser Pajuhi
3
Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
Lorestan University of Medical Sciences, Khorramabad, Iran.
Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
Background : Seizure is one of the most important symptoms of epilepsy and other neurological disorders. Satureja khuzestanica (SK) is an annual herb used in the traditional medicine of Iran. Because of adverse effect of synthetic medicines, nowadays medicinal plants are widely used in controlling convulsions. Convulsions investigated in mice.
Materials and Methods: In this experimental study, 40 male mice were studied. Different doses (40, 80, 120 mg/kg) of the essential oil were administered in groups 1, 2, and 3 and Carvacrol (80mg/kg)was administered in group 4. In the 3% Tween (equal volume with SKEO) were injected intraperitoneally. In all groups, after 30 min, Nicotine (5 mg/kg) was injected intraperitoneally and the time for onset, duration, and intensity of the convulsion were recorded.
Results: The results showed that the SKEO and Carvacrol delaye the onset of convulsion, and decreased the duration and intensity of convulsion in 80mg/kg doses.
Conclusion: The results of this study showed that SKEO has anti-convulsive effects. More investigations are needed in order to know the mechanisms of the extract.
http://yafte.lums.ac.ir/article-1-750-en.pdf
Satureja khuzestanica
Mice
Convulsion
Nicotine.
per
Lorestan University of Medical Sciences
Yafteh
1563-0773
2981-0779
2012-07
14
2
31
42
article
Purification and survey on the kinetic properties from Lathyrus Sativus histaminase via anion exchange chromatography
masoud Alirezaei
alirezaei_m54@yahoo.com
1
azadeh Khonsari
2
shima Neamati
3
najmeh Khalighyan
4
katayon Javidnia
5
Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Medicinal and Natural Products and Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Medicinal and Natural Products and Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Background : The histamine poisoning directly correlates to improper preservation of foods. Diamine oxidase catalyses the oxidative deamination of histamine and other biogenic amines such as cadaverine and putrescine. Plant diamine oxidases are of widespread occurrence in Leguminaceae such as Cicer arietinum, Lathyrus sativus and Vigna radiata. For this reason, this study was carried out to examine purification and enzyme kinetic properties of histaminase from the Lathyrus sativus seedling.
Materials and Methods: The purification of histaminase was according to the method given by Federico et al., with some modifications. In short, using ammonium sulfate precipitation of proteins, dialysis against potassium phosphate buffer 50mM, pH=7 over night, concentrating via dialysis bag and PEG, loading on DEAE52-sephadex column previously equilibrated with 15 mM potassium phosphate buffer pH=5.5 containing 1 µmol CuCl2 and finally enzyme eluted by increasing the NaCl concentration to 0.2 M. The homogenicity of enzyme was confirmed by SDS-PAGE in each step and diamine oxidase activity was determined by a spectrophotometer.
Results: The specific activity of the final purified enzyme was recorded at 50 U/mg protein for histamine substrate and enzyme kinetic properties of this enzyme (Km=2.5 mM, Vmax=3.05), and the homogenicity of purified enzyme by SDS-PAGE at 72 kDa region were indicated.
Conclusion: Overall, the herbal histaminase as a new enzyme in prevention of histamine poisoning is promising.
http://yafte.lums.ac.ir/article-1-751-en.pdf
Histaminase
Enzyme kinetic
Lathyrus sativus
per
Lorestan University of Medical Sciences
Yafteh
1563-0773
2981-0779
2012-07
14
2
43
49
article
The effect of Echinacea Purpura hydroalcoholic extract on the blood parameters in mice
mehrdad Modaresi
mehrdad_modaresi@hotmail.com
1
sedighe Asadi
2
Faculty of Agriculture, Islamic Azad University,Khorasgan Branch, Isfahan, Iran
Payam e Noor University, Isfahan, Iran
Background : Purple cone flower is a plant with scientific name Echinaceae purpurea that belongs to Echinaceae family. This plant has many pharmaceutical effects which one of the most important uses is Immunostimulating .This research attempts to study the effects of Echinaceae purpurea hydroalcoholic extract on the blood parameters in little laboratory female mice (Balb/c species) .
Materials and Methods: In this research, 48 mature female mice were divided randomly into 6 groups . The control group did not receive any extract , the placebo group received 0.5 cc normal saline every other day and 4 treatment groups received extract with (50,100,200,300) mg/kg/2day doses that was used interperitoneal injection .Then the blood samples were taken from the whole mice. The results were analyzed in the case that p<0.05,using SPSS software.
Results: The study of blood parameters showed that the extract increased markedly in the total number of white blood cells in each of 4 treatment groups in comparison with control group. The number of neutrophiles decreased significantly in each of 4 treatment groups in comparison with control group, but the significant difference wasn’t noticed in monocytes. The number of red blood cells increased significantly in groups 1 , 2 in comparison with the control group, but in groups 3 and 4 there was no significant difference. In this study, the extract had no statistical significant effect on the mean of hematocrit, hemoglobin and the index of red blood cells (MCV,MCH,MCHC).
Conclusion: The extract of Echinaceae purpurea can support the system of immunity by increasing the number of white blood cells and can be effective in hematopoiesis by increasing the number of red blood cells.
http://yafte.lums.ac.ir/article-1-752-en.pdf
Echinaceae purpurea
Blood parameters
Mice.
per
Lorestan University of Medical Sciences
Yafteh
1563-0773
2981-0779
2012-07
14
2
51
57
article
Phonological effects on chemical composition of essential oil of Satureja hortensis L.
ali akbar Tajali
tajali@iausr.ac.ir
1
babak Pilehvar
2
zahra Mirazadi
3
Faculty of Agriculture,Islamic Azad University, Shahre Rey Branch, Tehran, Iran
Faculty of Agriculture, Lorestan University, Khorramabad, Iran.
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Background : Summer savory is an annual grass plant, with a branched stem with the length of 10 to 30 cm that is distinguishable from the similar species due to having grayish green appearance and its leaves are narrow and long. In recent years, the anti-viral, anti-inflammatory, antibacterial, antifungal, antispasmodic, stomach invigorator and digestion facilitator characteristics of this species have been proved.
Materials and Methods: This study aimed to examine the type and amount of essence compounds in the summer savory was performed in two vegetative and reproductive phonological stages. To identify the amount of plant essence components a total of 30 samples were randomly taken from Shater Abad heights in two vegetative and flowering phonological stages and its essence was produced by Clevenger apparatus. GC/MS was used to identify essence constituents. In all spectra given by GC/MS, the Kovats index was calculated for each peak by the pattern of exiting the normal alkenes and spectrum inhibition index.
Results: The total amount of plant essence in two phonological stages before flowering (vegetative stage) and after flowering (reproductive stage) was 0/5 and 0/4 percent (w/v), respectively. The number of compounds identified in the vegetative stage was 27 compounds and for reproductive stages was 19 compounds upon which nearly 98% of plant essence were identified.
Conclusion: The results show that there is no significant difference in both vegetative and reproductive stages in terms of the type of main compounds of the summer savory essence, although the relatively large changes are seen in two phonological stages listed in terms of the numbers of identified compounds. Also the results obtained from this study showed that three main compounds of the summer savory essence are Thymol, gamma- Terpinene and Carvacrol, respectively, which their anti-bacterial, anti-fungal and antioxidant characteristics completely confirm the medicinal property and therapeutic effects of essence of this plant.
http://yafte.lums.ac.ir/article-1-753-en.pdf
Summer savory
Phonological stages
Thymol
Carvacrol
Gamma-Terpinene
per
Lorestan University of Medical Sciences
Yafteh
1563-0773
2981-0779
2012-07
14
2
59
69
article
Qualitative and quantitative comparison of essential oil of different ecotypes of Teucrium polium grown in Lorestan Province
ali Mohammadian
Mohammadian53@yahoo.com
1
reza Karamain
2
seyed peiman Hashemi
3
Lorestan University of Medical Sciences, Khorramabad, Iran,
Agricartural and Natural Resources Research Center, Khorramabad, Iran
Lorestan University, Khorramabad, Iran
Background : Teucrium polium is herbaceous, persistent and belongs to Lamiaceae family, Teucrium genus. Average plant height ranged from 10 to 40 cm. This plant contains compounds such as Terpenoide, Sapounin, Esterool, Flavenoied and Loukoantosianin and has antibacterial, but not significant antifungal, properties. Reduction in cholesterol, triglyceride and blood pressure, antioxidant, antifever, antipain, antiolseri and antispasmodic are among its medicinal properties.
Materials and Methods: In this study some flowering branches of different ecotypes of Teucrium polium were selected by random systematic method. The essential oil was extracted by distillated water using the Clevenger method. Chemical compounds of essential oil were identified by GC and GC/MS, and the obtained data were analysis by SPSS ver. 19.
Results: The data showed that there was significant difference between the amount of essential oil in different ecotypes of Teucrium polium, also there was significant difference(1%) between identified compounds into essential oils in different ecotypes. The main extracted compounds were :Germacrene- D, Farnesene-cis-b, -caryophyllene , Carvacrol,-pinene, bicyclogermacrene.
Conclusion: The results showed that four species of Teucrium were predominant in Lorestan province including: Teucrium orientale, Teucrium polium, Teucrium melissoides, Teucrium stocksianum. Also the results showed that Kharramabad ecotype was higher in oil yield (0.6%) and number of essential compounds. The main compounds extracted in Kharramabad were: cavacrol,bicyclogermacrene, -pinene, -caryophyllene, farnesene-cis-b. So Khorramabad ecotype is suggested as a priority for production.
http://yafte.lums.ac.ir/article-1-754-en.pdf
Essential oil
Teucrium polium
Ecotype
Lorestan province
per
Lorestan University of Medical Sciences
Yafteh
1563-0773
2981-0779
2012-07
14
2
71
80
article
Study of germination indices and characters and seed establishment of myrtus communis L.
ahmad Ismaili
1
hamid reza Eisvand
2
abdollhosein Rezaeinejad
3
kamran Sameey
4
seyed mahmod Zabeti
5
Faculty of Agriculture, Lorestan University, Khoramabad, Iran.
Faculty of Agriculture, Lorestan University, Khoramabad, Iran.
Islamic Azad University, Kangavar Branch, Kangavar, Iran
Faculty of Agriculture, Lorestan University, Khoramabad, Iran.
Background : Myrtle is a shrub and has a many applications in medicinal industries. Although the myrtle can reproduce by cutting, its reproduction by seed will bring more advantages such as economic advantages and better regeneration in nature or agriculture. Regarding to the difficult seed germination of this plant and also the need for extension the cultivation, this study was conducted for determination of germination inhibitor factors.
Materials and Methods: Mechanical scarifying (by sand paper) and chemical scarifying (by immerging the seeds in 98% sulfuric acid for 2 and 4 minutes and then 3 times rising in distilled water) were used against hardseeness (physical dormancy). Cold treatments (1 and 2 months) were used for physiological dormancy. All mentioned experiments were conducted based on randomized complete design with 3 replications.
Results: Study of healthy seeds showed the existence of a dual dormancy including mainly hardseedness (76%) and physiological dormancy in a part. Percent of seedling establishment was higher in mechanical treatment in compare of chemical ones and acid treatments also had some adverse effects on seedling establishment and increased the abnormality (especially on roots and vigor).
Conclusion: Separating the blank seeds and mechanical scarifying are the suggesting treatments to produce high seed germination and good establishment of seedling.
http://yafte.lums.ac.ir/article-1-755-en.pdf
Myrtle
Germination index
Seedling establishment
scarifying
Cold
Myrtus communis