72 1563-0773 Lorestan University of Medical Sciences 535 Amplification and flagellin typing of pseudomonas aeruginosa by molecular method goudarzi gholamreza yadegar abas c c Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University,Tehran,Iran 1 8 2011 13 2 1 5 05 11 2011 Pseudomonas aeruginosa possesses a polar flagellum made up of flagellin subunits that encoded by fliC gene. Flagellin from several strains of P. aeruginosa on the basis of reaction with polyclonal antibodies and apparent molecular weight was divided into a or b types. Material and Methods: After extraction of the genomic DNA from two standard strains 8821M (Type a) and PAO1 (Type b) and some clinical strains of P. aeruginosa, the full coding sequence of fliC was amplified by polymerase chain reaction (PCR) using the designed specific primers and its fragments length was compared. Results: The PCR and sequencing results showed that the length of fliC genes from the a and b strains were 1160 bp and 1460 bp respectively. Conclusion: This method could be utilized to determine flagellated (Motile) and non-flagellated strains of P. aeruginosa, genotyping, amplification of full coding sequence of fliC gene in order to clone and express recombinant flagellin protein.
534 The effect of Satureja Khuzestanica essential oil administration on intensity of Cisplatin induced nephropathy in rats rasoulian bahram d Javidnia marjan e Poladchang mina f Rashidipour marziye g delfan bahram h goudarzi samira i d Lorestan University of Medical Sciences, Khorramabad e Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran f Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran g Islamic Azad University, Khorramabad Branch, Young Researchers Club, Lorestan, Iran h Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran i Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran 1 8 2011 13 2 6 13 05 11 2011 05 07 2021 Cisplatin is a major chemotherapeutic drug in the treatment of some cancers and nephropathy is its main side effect. Reactive oxygen species have an important role in the nephrotoxicity of this drug. The goal of the present study was to investigate the effect of Satureja Khuzestanica essential oil, which contains antioxidant agents, on Cisplatin induced nephropathy. Materials and Methods: This study was performed on male 200-300gr Wistar rats. In control group Cisplatin (5mg/kg) was injected. In case groups, Satureja Khuzestanica essential oil with 50, 100 and 200mg/kg doses and then Cisplatin were injected. Also in some other groups essential oil and then normal saline (instead of Cisplatin) were injected. Three days after Cisplatin injection, plasma Creatinine and Urea levels and Creatinine clearance and fractional excretion of Na were measured as renal function tests. Results: Simultaneous administration of essential oil with a dosage of 200mg/kg and Cisplatin resulted in the death of animals. The essential oil injection with a dosage of 100mg/kg intensified nephropathy of Cisplatin and with a dosage of 50mg/kg the intensity of nephropathy did not have any significant difference with the control group. Essential oil administraton by itself had no effect on renal function. Conclusion: Simultaneous administration of Satureja Khuzestanica essential oil with Cisplatin not only will not decrease nephrotoxicity of this drug but also may increase the intensity of Cisplatin induced nephropathy in higher doses. 438 Improved survival of acute lymphoblastic leukemia patients of HLA-A3/11 absent for donor KIR3DL2 after non-T-cell depleted HLA-identical sibling hematopoietic stem cells transplantation shahsavar farhad j entezami kobra k Alimoghaddam kamran l j Lorestan University of Medical Sciences, Khorramabad, Iran k Tehran University of Medical Sciences,Tehran,Iran l Tehran University of Medical Sciences,Tehran,Iran 1 8 2011 13 2 14 25 23 07 2011 A potential factor influencing hematopoietic stem cells transplantation (HSCT) outcome is the presence of donor-derived alloreactive natural killer (NK) cells. This retrospective analysis studied the impact of NK alloreactivity based on the missing KIR ligand, for acute myeloid leukemia (AML) or acute lymphoblastic leukemia (ALL) patients undergoing non-T-cell depleted HLA-identical sibling HSCT. Materials and Methods: A total of 78 patients were studied, including 40 patients with AML and 38 patients with ALL. Nearly, all patients were received a uniform myeloablative conditioning regimen and prophylaxis for GVHD. All were genotyped for KIR genes and HLA ligands by means of polymerase chain reaction-sequence specific primers (PCR-SSP). Results: Missing KIR ligand without HLA-A3/11 had no effect on disease-free survival (DFS), overall survival (OS), or relapse in patients receiving transplants for AML or ALL. In patients with ALL, however, there was a significant missing KIR ligand with HLA-A3/11 effect on DFS (P=0.04) and OS (P=0.02). Conclusion: These data indicate that the absence of HLA class I ligand in the recipient for donor-inhibitory KIR can be a prognostic factor for transplantation outcomes in non-T-cell depleted HLA-identical sibling hematopoietic stem-cell transplantation and that the lack of HLA-A3/11 for donor KIR3DL2 can contribute to improved survival for patients with ALL. 439 The study of M.S patient's life style referred to MS association in Tehran city in 2008 Payamani firuzeh m nazari ali akbar n Noktehdan haydeh o mehran abas p Sahraian mohammad ali m Lorestan University of Medical Science n Tehran University of Medical Sciences,Tehran,Iran o Tehran University of Medical Sciences,Tehran,Iran p Tehran University of Medical Sciences,Tehran,Iran Tehran University of Medical Sciences, Tehran,Iran 1 8 2011 13 2 26 34 23 07 2011 13 10 2021 Multiple sclerosis is unpredictable and is amongst the important diseases which cause changes in life style and gradually leads to inability. There is a close relation between health and life style, so that one can prevent from rate of disease attacks, by his/her life style modification. Therefore recognition life style for educational planning in MS patients is very important. The aim of this study was to determine life style of MS patients referred to MS association in Tehran city in 2008. Materials and Methods: This descriptive cross-sectional study was carried on 200 men and women suffering from MS. Data gathering tool was a questionnaire and method was interview. Data were analyzed using descriptive and inferential statistics (Chi- Square and Fisher,s exact test) and SPSS software. Results: 38.5% of the samples in self care dimension were desirable. Findings also indicated that nutrition in the samples (78%) was relatively desirable. Other results showed that non smoking (83%) was desirable and sleep pattern and rest (45%), and stress coping (50.5%) was undesirable. Findings showed there was significant relation between educational level, supportive resources, number of relapse and self care dimension in life style. Also, relation between marriage status, family income and nutrition dimension in life style was significant too. Conclusion: According to the results of this study, life style in some dimensions was undesirable. These non healthy behaviors can effect on severity and relapse of disease and ultimately have bad influence on the quality of life in these patients. Therefore, change and modification and improvement in life style in these patients seem to be essential that we can access to it with comprehensive educational programs. 440 Kinetic and isotherm study of hexavalent chromium removal from aqueous solutions using calcium alginate beads Shams Khorramabadi ghodratollah Darvishi Cheshmeh Soltani reza Rezaee abas Jonidi Jafari ahmad Department of Environmental Health, Faculty of Health, Lorestan University of Medical Sciences,Khorramadab,Iran. Department of Environmental Health, Faculty of Medical Sciences, Tarbiat Modares University Department of Environmental Health, Faculty of Health, Tehran University of Medical Sciences Department of Environmental Health, Faculty of Health, Tehran University of Medical Sciences 1 8 2011 13 2 35 45 26 07 2011 22 06 2021 One of the most important environmental problems is water and soil contamination with heavy metal ions. In this direction, the aim of this investigation was biosorptive removal of Cr (VI) using calcium alginate and determination of isotherm and kinetic of biosorption. Materials and Methods: Using batch system, biosorption capacity of calcium alginate for removal of Cr(VI) ions was investigated as function of pH, shaking time and Cr(VI) ions concentration. For determination of active bands and biosorbent characteristic, Fourier Transform Infrared (FT-IR) and Scanning Electron Microscopy (SEM) analysis were carried out respectively. Results: Regarding the FT-IR analysis, It was shown that C=O and O–H groups played a significant role in Cr (VI) biosorption onto alginate calcium. The optimum pH was obtained at pH value of 4.0. Suitable fitness between the experimental data and studied isotherm was obtained (R2>0.9). Maximum biosorption capacity of calcium alginate was 84.75 mg/g. The mean free energy of Cr(VI) biosorption (E) was calculated 9.129 kJ/mol. Moreover, the pseudo-second order kinetic model was found to be suitable than the pseudo-first order kinetic model to correlate the experimental data (R2>0.97) and equilibrium was achieved within 120 min. Conclusion: It can be stated that biosorption of Cr (VI) onto calcium alginate occurs through a chemical mechanism. Also, according to maximum biosorption capacity, it can be stated that calcium alginate is an effective and efficient biosorbent. 441 The comparison of designed slipper splints with the splints available on the market in the treatment of hallux valgus Mirzashahi babak Ahmadifar mahdiye Birjandi mehdi Pournia yadollah Lorestan University of Medical Sciences, Khorramabad, Iran Khorramabad Islamic Azad University Department of Biostatistics, Lorestan University of Medical Sciences, Khorramabad, Iran Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran 1 8 2011 13 2 46 53 26 07 2011 20 06 2021 Hallux valgus or laterally deviation of thebig toe to the out side is a complex disease. If there is not any treatment it will cause deviation of other toes. Hallux valgus is 3 times more common in females that may cause uncomfortable deformity of foot , problem with wearing unsuitable and narrow-toed box shoes and pain on the inside of the big toe over the metatarsal joint , therefore patients may refer to physician. Untreated Hallux valgus may cause hamer toe deformity of the second toe. Materials and Methods: This study was a cohort study carried out on the patients referred to orthopedic clinic of Ashayer hospital with complaint of Hallux valgus. Two groups of 30cases were ramdomly selected and then the splints designed by us were given to case group and Night splint and interdigital pad were given to the control group. The patients were followed for one year as every three months and in every time weight bearing antroposterior radiography of both foot were taken and Hallux valgus and inter metatarsal angles were measured, and data were analyzed using SPSS and repeated measure test. Results: In the patients who used our splints regularly Hallux valgus angles decresed more considerably than those who used splints available on the market ( p<0.001). Conclusion: This study showed that despite the contraversies in nonoperative treatment of Hallux valgus, if the Hallux valgus angle of patients are mild to moderate, this splints can be used to treat it. 442 Antioxidant effects of aqueous fruit extract of Ziziphus jujuba on ethanol-induced oxidative stress in the liver and kidney of male rats Taati majid Alirezaei masoud Meshkatalsadat mohammad hadi Rasoulian bahram Dezfolian omid Neamati shima Department of Physiology, Faculty of Veterinary Medicine,Lorestan University, Khorramabad,Iran. Department of Biochemistry, Faculty of Veterinary Medicine,Lorestan University, Khorramabad,Iran Department of Chemistry, Faculty of Basic Sciences,Lorestan University,Khorramabad,Iran. Razi Herbal Medicines Research Center,Lorestan University of Medical Sciences,Khorramabad,Iran. Department of Pathology,Faculty of Veterinary Medicine,Lorestan University,Khorramabad,Iran. Razi Herbal Medicines Research Center,Lorestan University of Medical Sciencas,Khorramabad,Iran 1 8 2011 13 2 54 68 26 07 2011 22 06 2021 It is well known that pathogenesis of ethanol in liver and kidney is directly related to increase of free radicals and oxidative stress which lead to the structural and functional damage in these two vital organs. Materials and Methods: Fifty-six male Wistar rats were randomly divided into eight identical groups and were treated as follows: control group (normal saline), the ethanol-fed group (4 g/kg body weight), 3 groups received three doses of Ziziphus jujuba fruit extract (50, 100, 200 mg/kg body weight) and 3 groups were fed with three doses of Ziziphus jujuba fruit extract plus ethanol (50, 100, 200 mg/kg plus 4 gr /kg body weight). All treatment were applied once daily by gastric gavage for 60 consecutive days. Samples of serum, liver and renal tissues were achieved for analysis of serum components, antioxidant enzymes, and thiobarbituric acid reactive substances (TBARS) from rats. Results: Superoxide dismutase and glutathione peroxidase (GPx) liver activities were decreased significantly in ethanol group compared to the control group. While, only glutathione peroxidase activity of liver increased significantly by administration of Z.jujuba fruit extract (200 mg/kg). The activity of kidney GPx, increased significantly in ethanol group compared to the control group. Also, kidney GPx activity decreased significantly in Z.jujuba fruit extract and ethanol group (200 mg/kg plus 4gr/kg) in comparison with the control group. In the present study, liver TBARS concentration, indicator of lipid peroxidation, increased significantly in ethanol group compared to the control group (p<0.05). Conclusion: Pretreatment by Z.jujuba fruit extract can protect liver and kidney against ethanol–induced oxidative stress. 443 Study of frequency of operated chest wall tumors In Al Zahra hospital from 2007 to 2009,Isfahan,Iran Tabatabai seyed abas Hashemi seyed mozafar Ahmadi Nejad mojtaba Toolabi aram Afshar Pour sadi Department of Surgery,Isfahan University of medical sciences, Isfahan, Iran. Department of Surgery,Isfahan University of medical sciences, Isfahan, Iran. Department of Surgery ,Lorestan University of Medical Sciences, Khorramabad, Iran Lorestan University of Medical Sciences Khorramabad, Iran 1 8 2011 13 2 69 73 26 07 2011 20 06 2021 Chest wall masses are a broad spectrum of bening or malignant growth of cells.Different studies reported different results about chest wall damage,but there is not holistic information about different types of thoracic damage in patients yet.The aim of this research is to study frequency of operated chest wall tumors in Isfahan Al Zahra hospital based on epidemiologic variables, how to respond to the treatment and the fate of these Patients. Materials and Methods: This descriptive cross-sectional study was conducted in Al Zahra training and treatment center in 2009. The sampling method was census. The records of all patients who underwent surgery from 2007 to 2009 were studied. The data were collected by a special questionnaire and analyzed by SPSS software. The Chi-square and T-student tests were used for data analysis. Results: 112 records of the patients with chest wall tumors were studied that 46 cases(41.1%) were benign and 66 cases(58.2%) were malignant. The frequency distributions of malignant tumors in males and females were 56.5% and 62% and the difference between two sexes was not statistically significant (P= 0.55). Conclusion: According to the results of this study about 59% of the chest wall tumors were malignant and in the case of being hesitant about existing a mass on the chest wall, needed measurements for treatmentand and on time removal of the mass must be done. 444 The effect of hydro – alcoholic seeds extract of Ceratonia siliqua L. on the kidney functional factors and serum electrolytes in diabetic male rats Mokhtari mokhtari Mohammadi behzad Sharifi esfandiar Shahamir Tabatabaee maryam Department of Biology,Islamic Azad University,Kazerun Branch,Kazerun, Iran Islamic Azad University, Kazerun Branch,Kazerun, Iran Department of Biology, Islamic Azad University, Kazerun Branch,Kazerun, Iran. Department of Biology, Islamic Azad University, Kazerun Branch,Kazerun, Iran. 1 8 2011 13 2 74 82 26 07 2011 12 07 2021 Diabetes Mellitus is the most important human metabolic disease which leads to hyperglycemia and hyperlipidemia. Hyperglycemia can give rise to disorder in kidney. In the present study, we attempted to investigate the effect of hydro-alcoholic seed extract of Ceratonia siliqual. on the blood levels of creatinine,uric acid,BUN,Na+,K+and Ca2+in diabetic male rats. Materials and Methods: In this experimental study, fifty-six adult male Wistar rats each weighing 210-220g was randomly divided into seven groups of eight. These groups include: control group which left untreated sham group which received distilled water treated control group received only 600mg/kg seed extract diabetic control group getting only Streptozotocine and three experimental groups 1, 2 and 3 receiving 150,300 and 600mg/kg extract respectively. Diabetes was induced by intraperitoneal injection(60mg/kg) of STZ. Following induction of diabetes, the extract dosages were administered to the rats by gavageing method for sixteen days. Serum levels creatinin,uric acid,BUN,Na+,K+and Ca2+were measured by outoanalyzer. The data were analyzed by SPSS-18 software using ANOVA test. The results were expressed as Mean±SEM and a P≤0.05 was set as significant value. Results: In experimental group 1 concentration of creatinine and BUN decreased significantly, while K+ level was declined in experimental groups 1 and 3. The serum level of uric acid in experimental group1 showed a considerable elevation. In addition, in experimental groups 1 and 3 concentration of Na+ increased significantly. There were no significant differences in Ca2+ concentration among various groups. Conclusion: Ceratonia siliqua seed extract can improve distractive effects of diabetes on kidney. 445 Evaluation of sensitivity of three different primers using PCR- RFLP method for molecular differentiation of atypical mycobacteria Heidari feze Farnia parisa norozi jamile majd ahmad Islamic Azad University, Tehran North Branch, Tehran, Iran Mycobacteriology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Department of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, Iran Department of Biology, Islamic Azad University, Tehran North Branch, Tehran, Iran 1 8 2011 13 2 83 91 26 07 2011 13 10 2021 i Rapiddentification of atypical mycobacterium(Non-tuberculosis mycobacterium NTM) is important because of increase of these organisms infection in recent years. As phenotypic tests are time consuming and expensive, nowadays the molecular methods are widely used for rapid identification of mycobacterium spcies. The aim of this study was molecular differentiation of atypical mycobacteria using three primers in the PCR- RFLP method and evaluation of sensitivity of primers. Materials and Methods: This study was performed on 48 atypical mycobacterium specimens separated from pulmonary tuberculosis (PTB) patients who were indentified by phenotypic tests. Drug susceptibility testing was performed by proportional method, the fragments of the 16S-23S rRNA gene spacer and hsp65 gene were amplified by PCR method. Subsequently the amplicons were digested with enzymes namely AvaII,HphI, HpaII, BstEII and HaeIII and electrophoresed on 2% agarose gel. Results: A total of 48 isolates, 8 (16.6%) had multi-drug resistant (MDR-TB), 4 (8.3%) had susceptible and 36 (75%) had non MDR ( combined resistance). 13 (27%) were rapid growing and 73% were slow growing . By rate detection of hsp65 PCR-RFLP primer was higher than other primers. Conclusion: hsp65 PCR- RFLP method was more specific and exact for differentiation of non tuberculosis mycobacterium(NTM). 446 Diagnosis and treatment of antisperm antibody Kheirollahi abolreza Shahsavar farhad Sabooteh tomaj Department of Urology, Faculty of Medicine, Lorestan University of Medical Sciences,Khorramabad, Iran. Department of Immunology, Faculty of Medicine, Lorestan University of Medical Sciences Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran 1 8 2011 13 2 92 104 26 07 2011 Immunoinfertility because of antisperm antibody (ASA) is one of the important causes of infertility in humans. The incidence of ASA in infertile couples is 9-36% depending on the reporting center. The prevalence of ASA is 8-21% in the infertile male partners and 6-23% in the infertile female partners. There are several methods to detect ASA. In the past, the clinical interest in ASA was hampered by the fact that a standardized assay for the detection of ASA was lacking. However, it has to be clarified whether each antibody binding to an antigen, which is identified on the sperm surface, also influences sperm function. Several methods have been reported for treatment of immunoinfertility. Most of the available techniques have side effects, are invasive and expensive, have low efficacy, or provide conflicting results.This review article will help to increase our knowledge about diagnosis and treatment methods of ASA. 447 Allgrove (AAA) Syndrome manifestations after gastroenteritis: A case report Taee nadereh Abbassi farzaneh Tarhani fariba Department of Pediatrics,Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad ,Iran Department of Endocrine and Pediatric Metabolics, Tehran University of Medical Sciences,Tehran, Iran Department of Pediatrics,Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad ,Iran 1 8 2011 13 2 105 111 26 07 2011 13 10 2021 Allgrove (AAA) syndrome or familial glucocorticoid deficiency or Tripple A Syndrome is a rare genetic disorder with transmitted autosomal recessive. Allgrove and colleagues first described this syndrome in 1978. Allgrove Syndrome characterized by Adrenal insufficiency, Alacrima and Achalasia. Neurological and dermatological findings may be presented in some patients. Hyperpigmentation and Alacrima are diagnostic key findings in Allgrove Syndrome. In this report we describe a 2 year old patient with the clinical picture of Allgrove Syndrome after gasteroenteritis.