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Vali-e-Asr University, Rafsanjan
Abstract:   (118 Views)
Background and Aim: Due to the decrease in muscle mass during reduced physical activity and the role of TRPC1 and TRPC3 in some models of muscle atrophy, the aim of the present study was to investigate the effect of nerve chronic constriction injury (CCI) on the expression of TRPC1 and TRPC3 genes in male wistar rat plantaris muscle after a period of endurance training.
Materials and Methods: Thirty-two 8 week-old rats (250±20 g) were randomly divided into four equal groups. 1- Control group, 2- Control-CCI group (three branches of their sciatic nerve were tightly tied), 3- Training-CCI group (after a period of endurance training, three branches of their sciatic nerve were tightly tied) and 4- Training group (only participated in endurance training program). At the end of the study, the rats were anesthetized using CO2 gas and their plantaris muscle was extracted. Gene expression was measured by Real-time PCR. Two-way analysis of variance was used to analyze the data.
Results: There was a significant decrease in the weight of plantaris muscle between the control-CCI group and other groups (P=0.001). There was a significant increase in TRPC1 gene expression between the training group with the control-CCI and control groups (P=0.001) and also between the training-CCI group with the control-CCI and control groups (P=0.006). Also, there was a significant increase in TRPC3 gene expression between the training group and other groups (P=0.001). In addition, there was a significant increase between the training-CCI group and the control-CCI group (P=0.008).
Conclusion: CCI leads to muscle atrophy and decreased expression of the TRPC1 gene, and endurance training leads to increased expression of the TRPC1 and TRPC3 genes.
 
     
Type of Study: Research | Subject: قلب و عروق
Received: 2022/04/7 | Accepted: 2022/07/1

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