Background: free radicals produced as a result of heavy training exercise especially oxygen species (ROS) damage to body tissues Which can be prevent from this by consuming antioxidant substances timely. The aim of this study was to evaluate effect of Pomegranate (Punica Granatum) peel hydro alcoholic on reduced of oxidative stress induced by an exhaustive exercise.

Materials and Methods: Thirty two weight-matched male Wistar rats were evenly divided into: 1) control: received saline (0.2 ml saline/ rat) by oral administration via epigastric tube. 2) Received oral administration of 200 mg/kg pomegranate peel hydro alcoholic extract (PPHE200). 3) Received oral administration of 250 mg/kg Pomegranate peel hydro alcoholic extract (PPHE250). 4) Received oral administration of vitamin E (vit E) 5 mg/kg. Animals were submitted to swimming exhaustive exercise stress for an 8-week. At the end of the experiment, blood samples were collected for serum. Serum samples were analyzed for paraoxonase-1(PON-1) and myeloperoxidase (MPO) activities and nitric oxide levels.

Results: Paraoxonase-1 (PON-1) activities serum were significantly increases in PPHE200 (23.03±1.47), PPHE250 (23.59±1.98) and vit E (25.38±2.65) than in the control (18.57±1.380) (p<0.05).In PPHE200 (32.76±9.97) ،PPHE250 (31.45±6.05) and vit E (24.94±4.65) treated animals was determined in serum where myeloperoxidase activities reduced significantly compared with control (40.70±6.14) (p<0.05). Levels of Nitric oxide levels were significantly lower in PPHE 200 (46.59±2.48), PPHE250 (40.27±2.62) and vit E (36.25±3.82) treated than in control (47.18±5.36) (p<0.05).

Conclusion: Results indicated that Pomegranate peel hydro alcoholic extract supplementations can strength antioxidant defense system and anti-inflammatory induced by exhaustive exercise.