Showing 5 results for Asgari
Hasan Hosain Zadegan , Morteza Salari, Abdol Amir Allame , Zahir Mohammad Hasan, Simin Asgarian ,
Volume 5, Issue 4 (1-2004)
Abstract
مقدمه: وروتوکسین ها سمومی با ساختمان دو بخشی هستند که در پاتوژنز سویه های اشرشیا کولی انتروهموراژیک دخالت دارند. مطالعات اولیه درمان برخی تومورهای سرطانی و نیز بررسی اثر سیتوتوکسیسیته این سموم روی رده های توموری در شرایط محیط کشت (In Vitro) وحیوانات آزمایشگاهی نشان دهنده اثر بخشی آنهاست.این سموم به دلیل دارا بودن گیرنده های اختصاصی بر روی رده های توموری مطالعه شده به صورت انتخابی عمل می نمایند.
هدف از این تحقیق بررسی سیتوتوکسیسیته وروتوکسین1 تخلیص شده از سویه مولد وروتوکسین اشریشیا کولی توأم با منوفسفوریل لیپیدA (MPL) بر روی رده سلولی تومور پستانی انسان به نامMCF-7 بود.
مواد و روشها: در این تحقیق ابتدا وروتوکسین1 تولید شده از 5 سویه مولد وروتوکسین به دست آمده از انستیتو پاستور ایران و آزمایشگاه مرجع بو علی با استفاده از کیت آگلوتیناسیون معکوس (VTEC-RPLA) تأیید و با استفاده از کروماتوگرافی جذبی تخلیص شد. در مرحله بعد رقت های مختلف وروتوکسین1 و MPL به صورت توأم و یا جداگانه بر روی رده سلولی MCF-7 اضافه شد و قابلیت زنده ماندن سلول ها Viability) ( با استفاده از تریپان بلو و شکستگی ژنوم آنها با الکتروفورز آگارز بررسی گردید.
یافته ها: مطالعات ما نشان داد که سلول های MCF-7 مطابق با تحقیقات قبلی حساسیت فوق العاده ای به وروتوکسین1 دارند و مقدار 33 نانوگرم در هر میلی لیتر اثری معادل دوز سیتوتوکسیک 50 درصد CD50% ایجاد می کند. بررسی های شکستگی ژنوم سلولی الگوی مشخصی از بروز پدیده آپوپتوزیز را نشان می دهد. در حالی که MPL به تنهایی ماده ای غیر سمی است؛ ولی استفاده توام آن با وروتوکسین1 اثرات سمی آن را تشدید می کند.
نتیجه گیری: اگرچه اثرات ضد توموری وروتوکسین1 از سال ها قبل روشن شده است؛ ولی کشف و شناسایی عوامل مختلف تشدید کننده اثر سیتوتوکسیک مواد ضد تومورها نیز مورد توجه محافل علمی است.MPL یکی از متابولیت های میکروبی است که اثر سینرژیستی آن با وروتوکسین1 در مرگ سلول های توموری پستان برای اولین بار در این تحقیق مورد بررسی شده است.
Ghorban Asgari , Hosein Movahdian , Bizhan Bina ,
Volume 5, Issue 4 (1-2004)
Abstract
Introduction: Bioassay is necessary for assessment of pollution. Because physical and chemical taste are not enough for assessment of potential effects on water ecosystem. Bioassay tests have alot of scientific value inanition doing of them is easy and need less time, also need less space, laboratory equipment’s and expenditure. Therefor such tests have found special attention in water pollution control measure. So whit determine of effluent toxicity and survey of its effects on water ecosystem by bioassay method, can determine the degree and the kind of treatment that are necessary to having optimum condition for water ecosystem. In this research survey of indicator toxicity of Isfahan south wastewater treatment by daphnia magna has been studied.
Materials and methods: In this study experiments were done on samples of wastewater that has been taken from Isfahans south wastewater treatment (samples has been taken from four place, influent raw wastewater, before and after primary settling tank and after secondary settling tank). Primary daphnia were couch from there living sites, then one of them were cultured alone, after infants of primary daphnia were used for culture in large amount. After prepering necessary dilution of wastewater samples by doing necessary experiments LC50-48-h toxic unit, efficiency of different units and total of wastewater treatment plant were determined.
Findings: Results reveal that LC50-48-h of influent wastewater is 30%(v/v) and toxic unit is 3.33 and LC50-48-h of wastewater before primary (after preliminary treatment) settling tank, after primary settling tank and after secondary settling tank was determined 32%, 85% and 32% (v/v) respectively, that was on the basis acute toxic unit (ATU) 3.125, 1.92, and 1.76 respectively, efficiency of preliminary treatment in reducing toxicity were determined 6% and efficiency of primary treatment secondary treatment and whole of treatment plant in reducing toxicity were determined 38.4%, 8% and 50% respectively .
Conclusion: Data of this research show that bioassay are necessary for assessment of wastewater and control of water pollution. So bioassay can be used as a suitable method for assessment of effect and efficiency of different treatment units and control of toxicity as base information for monitoring of effluent and due to increasingly developing of wastewater treatment systems in Iran, that is necessary appropriate strategy be under taken to monitoring the effluent . The result of this research and same research can be useful.
Ghorban Asgari , Bahram Kamarehei,
Volume 11, Issue 1 (yafteh 2009)
Abstract
Background: The consumption of aquatic nutrition sources, especially fishes, considered as a partial supply of protein, for ever-increasing demand of protein. According to statistics, the annual consumption of aquatic sources per capita in the word increased from14. 3Kg in the year1994 to more than 15. 75 Kg in 1997 and in Iran it increased from less than 1 kg in the year 1978 to 4.57 kg in the year 1996. So an ever-increasing demand of protein sources of the food and bioaccumulation of toxic compound especially toxic heavy metals increase safety control importance of the food. In this descriptive cross-sectional study heavy metals concentration Cd, Cr, Pb and Ni in cultured ponds fishes of Khorramabad city were studied in 2006.
Materials and Methods: To evaluate the safety of the cultured ponds fishes in khorramabad city from toxic heavy metals Cd, Cr, Ni and Pb, 240 samples of fishes were collected. The fishes were collected in the Spring and Summer seasons in 2006. The samples washed and digested with acid before using Atomic Absorption for determination of the metals, then data were analyzed using t-test and SPSS software.
Results: The results of the study showed that the average value of Lead, Chromium, Cadmium and Nickel in ten cultured ponds fishes tissue were 0. 46, 0. 06, 0. 053 and 0. 30 ppm (dry weight basis) respectively The study also showed that the amounts of Pb in 27%, Cd in 8%, Cr in 3% and Ni in 25% of the samples exceeded the WHO standard limits while the averages were blow the standard.
Conclusion: The statistical analysis of the samples showed that there are not significant differences between the different species of the fishes for all the heavy metals, except the fishes collected from the cultured ponds. Higher amount of the heavy metals in sea fishes could be related to fish food, which could bioaccumulated in fish tissues. The study revealed that non of the heavy metals collected in the fish samples exceeded the WHO standards.
Bahram Kamarehei , Seyed Hamed Mirhosseini , Ali Jafari, Ghorban Asgari , Mehdi Bierjandi , Zeinab Rostami,
Volume 11, Issue 4 (yafte 2010)
Abstract
with industrial and economic growth and different material production that humans gained from natural resources for their comfort and walfare, inwardly introduced toxic material and heavy metal entered environment that there created serious problems for themselves and environment. This study accomplished to determine heavy metal concentration (As, Ba, Cd, Hg, Pb, Cr)in water resources and river of Borujerd city in 2008-2009. Materials and Methods: This descriptive cross-sectional study was conducted to determine heavy metal concentration (As, Ba, Cd, Hg, Pb, Cr) in water resources and river of Borujerd city. 54 samples of water were taken from 18 drinking water wells, and also in two times 8 samples of Borujerd river were taken from before and after the city. Then samples carried into the lab and were concentrated ten times using expressed methods and standard methods. Then heavy metal concentration determined by AAP (WFX 130) and results analyzed by SPSS and EXCEL software. Results: Heavy metal concentration average (As, Ba, Cd, Hg, Pb, Cr) in drinking water wells were 0. 0, 0. 3222, 0. 0014, 0. 0002, 0. 0077 mg/l respectively. and heavy metal concentration in river water after the city has been increased than before the city. Conclusion: Results indicated that heavy metal concentration average in Borujerd drinking water wells were lower than standard amounts and drinking water wells didn’t pollute with heavy metal. But heavy metal concentration in river water after the city has been increased than the before of it because city waste water enters the city river.
Hedayat Nazari , Ali Farhadi , Mojgan Jariayani , Reza Hosseinabadi , Sara Asgari , Majid Majidimehr ,
Volume 16, Issue 2 (9-2014)
Abstract
Background : Many physical and psychological changes occur in women during pregnancy and this period has been conceptualized as a time of vulnerability for women . The present study aimed at determining the mental health status of pregnant women .
Materials and Methods: In this descriptive cross-sectional study, 280 pregnant women serviced by Khorramabad health care centers were assessed. Random sampling method was used to select the health care centers, and the women were selected by systematic sampling. The research instrument was the standard GHQ questionnaire. Then the data were analyzed by one way variance analysis and t test using the SPSS software .
Results: The findings showed that 43.6%of the pregnant women were suspected in terms of mental health. The highest frequency was found for social function (7.81) and the lowest belonged to depression (2.86).There was a significant relationship between mental health of the pregnant women with unwanted pregnancy and gender of fetus. Furthermore ,anxiety in the women in the third trimester of pregnancy was significantly higher than those in the first trimester of pregnancy (p<0.05).
Conclusion: Regarding the importance of mental health of pregnant women and its effects on fetus and newborn, integration of psychological counseling in prenatal care programs is recommended .
