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PCR identification of Pseudomonas aeruginosa based on two outermembrane lipoprotein oprI, oprL, and exotoxin A gene
Mohammad Mehdi Aslani , Marjan Hahsemipour , Vajihe Sadat Nikbin , Fereshte Shahcheraghi , Akram Eidi , Zeinab Sharafi ,
Volume 11, Issue 2 (yafteh 2009)
Abstract

Background: Pseudomonas aeruginosa has emerged as one of the most important nasocomial pathogen resulting in morbidity and mortality rates.

The aim of this research was to PCR identification of P. aeruginosa isolated from tracheal samples based on the amplification of I lipoprotein (oprI) for detection of genus and L lipoprotein (oprL) for detection of species and Exotoxin A (toxA) gene.

Materials and Methods: A total of 120 P. aeruginosa clinical isolates were collected from patients with tracheal infections. Chromosomal DNA of the isolates was extracted and used for PCR of oprI, oprL and Exotoxin A (toxA).

Results: From 120 P. aeruginosa 100% were positive for oprI and oprL genes based on PCR assay and from these samples 83% were positive for ExotoxinA (toxA).

Conclusion: Using PCR method with genome and specific primers can be more accurate and sensitive than biochemical tests to identify P.aeruginosa strains. It is also replaceble method for biochemical methods.

According to the results obtained by PCR test toxA gene is not as sensitive as oprI and oprL to recognize P. aeruginosa. So using PCR of all these three genes are necessary to detecte this bacterium.


Objectives, Outcomes, and Obstacles of Using Metaverse in the Field of Telemedicine: A Systematic Review
Mohamad Medi Askarizade, Nasim Aslani, Parastoo Amiri, Taleb Khodaveisi, ,
Volume 27, Issue 1 (12-2025)
Abstract
Background: Today, we can benefit from the capabilities provided by Metaverse in the methods of providing healthcare services, especially telemedicine. This requires an early assessment of the potential benefits and challenges of Metaverse in the field of telemedicine before clinical adoption is realized. The purpose of this research is to identify the goals, consequences, and obstacles of using Metaverse in telemedicine.
Materials and Methods: In this systematic review, we searched the articles published in online databases (PubMed, Scopus, and Web of Science) until June 2024 (without time limitation) using relevant keywords ("Metaverse" and "Telemedicine") in English. Exclusion criteria included articles that did not focus on this topic.
Results: Of all the articles included in the study, most of the studies (75%) were conducted in 2023, and South Korea accounted for the highest number of studies (25%) in this field. The main capabilities and consequences of using Metaverse in telemedicine included increasing access to healthcare services, increasing the level of security, creating a sense of confidence for users, reducing costs, and managing time. Risks related to security and confidentiality, required technologies, knowledge of use, and cost are also challenges and obstacles to implementing Metaverse in telemedicine.
Conclusion: Metaverse is a suitable platform for telemedicine that provides access to medical and scientific information. Despite the advantages and positive consequences of this platform in providing fast and quality medical services, which causes widespread attention, there are challenges in its implementation that need to be solved by necessary measures.
 

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