Yafteh

---

Background: Pseudomonas aeruginosa has emerged as one of the most important nasocomial pathogen resulting in morbidity and mortality rates.

The aim of this research was to PCR identification of P. aeruginosa isolated from tracheal samples based on the amplification of I lipoprotein (oprI) for detection of genus and L lipoprotein (oprL) for detection of species and Exotoxin A (toxA) gene.

Materials and Methods: A total of 120 P. aeruginosa clinical isolates were collected from patients with tracheal infections. Chromosomal DNA of the isolates was extracted and used for PCR of oprI, oprL and Exotoxin A (toxA).

Results: From 120 P. aeruginosa 100% were positive for oprI and oprL genes based on PCR assay and from these samples 83% were positive for ExotoxinA (toxA).

Conclusion: Using PCR method with genome and specific primers can be more accurate and sensitive than biochemical tests to identify P.aeruginosa strains. It is also replaceble method for biochemical methods.

According to the results obtained by PCR test toxA gene is not as sensitive as oprI and oprL to recognize P. aeruginosa. So using PCR of all these three genes are necessary to detecte this bacterium.

---

Hyperlepidemy or the increase in the rate of blood lipids is a condition which is developing as an illness in new decades. With regard to problems as a result of using drugs and also benefits of physical activity to improve hyperlepidemia, this research has tried to study the effect of the physical activity with regard to energy expenditure on blood lipids. Materials and Methods: To do the study, 21 hyperlepidemic men were selected and received a training protocol (the physical activity with 450 and 600 kcal). Results: Results showed that burning 450 kcal per season caused a significant change at levels of total cholesterol and HDL (P≤0/05). However to cause significant changes at levels of the triglyceride need to burn 600 kcal energy expenditure per season (P≤0/05). No significant change was found for both groups in relation to LDL levels and so can be concluded that, burning 600 kcal per session is not enough to cause change at LDL levels. Conclusion: In general, results showed that to cause positive changes at levels of HDL, less physical activity is needed, but to cause positive changes at levels of triglyceride, physical activity and energy expenditure should be increased.

---

Background : Obesity and overweight are related to biological factors, individual, social, economic factors, different cultural factors and feeding behavior.  Recognizing these factors can be a guidance of interventions relating to prevention and control of obesity in children and teenagers. The present study was aimed to study determinant factors of obesity and overweight in students of Khorramabad city.

Materials and Methods: In this cross-sectional study, 3387 students from age 6 to 14 were selected by combinative, multistage grouped and clustered sampling. Students' height and weight were measured and also body mass index (BIM) was calculated. In order to determine  the  prevalence, body mass index was used  for age in NCHS table and evidence up 95 was regarded as obese and 85 to 95 was regarded as overweight.    

Results: The relation of overweight and obesity in students with kind of school, family size, parental education and family income was very significant     (p<0.001). Obesity and overweight were more prevalent in students of non-profit schools and families with more income. Students whose parents had higher education, the prevalence of overweight and obesity was more. There wasn't  a significant relation between overweight and obesity and pubertal status (p=0.1).

Conclusion: Overweight and obesity in case study students were related to individual and external factors. Of these factors, we can mention factors such as gender, birth rank, family size and parental education.