Showing 5 results for Esmaeil Lashgarian
Kiyana Shahzamani, Hamed Esmaeil Lashgarian , Omid Ali Papi, Hamid Mokhayeri,
Volume 18, Issue 1 (5-2016)
Abstract
Background : Molecular diagnostic methods are among major tools in management of hepatitis C virus (HCV) in infected patients. Many studies have shown that viral load is associated with stage of infection and response to treatment. Therefore, the evaluation and quantification of viral load is very important. The goal of this study is implementation of inexpensive, yet accurate method for quantitative assessment of viral load in plasma samples of infected patients.
Materials and Methods: After development and validation of the assay, quantification of HCV RNA on 200 chronic patients the start of therapy was performed using an In-House Real-time PCR assay. Measuring the concentration of viral RNA was performed using an external standard curve. It should be noted that the validation and standardization of all procedures in this study were performed using RNA standard panel. The results of this method were compared with results obtained from Artus commercial kit.
Results: Detection limit of the assay was 50 IU/ml. The mean viral load measured on a logarithmic scale (5/81± 0/22, p<0/05). Parallel analysis of samples with commercial kit showed that there is a good correlation between these two methods (R2 = 0.988 p< 0.05).
Conclusion: Viral load detection of HCV was reported for the first time in Khorramabad city. According to the results, this method has a good sensitivity and specificity for HCV quantification in large-scale. It can be a good replacement for commercial kits especially for clinical evaluation of therapy.
Saeid Pirmoradi, Hassan Dariushnejad, Vajihe Ghorbanzadeh, Hamed Esmaeil Lashgarian,
Volume 21, Issue 4 (1-2020)
Abstract
Tumorigenesis is a complex and dynamic process. The microenvironment of tumors plays a pivotal role in the formation, initiation and progression of tumors. Studies on neoplastic developments focusing on tumor microenvironment events demonstrate its significance in promoting tumor progression. Prior to tumor metastasis, primary tumors secrete factors that create a proper metabolic environment for tumor metastasis. These conditions prepare the microenvironment for better and more efficient tumor growth. On the other hand, tumor cells continuously interact with the surrounding microenvironment, and targeting the tumor microenvironment can complement traditional treatment and improve the therapeutic outcomes for these malignancies. In the present article, the researchers attempt to highlight the importance of tumor microenvironment in tumorigenesis and tumor metabolism.
Seyede Maryam Valizadeh Otaghsara, Vajihe Ghorbanzadeh, Hamed Esmaeil Lashgarian, Pejman Hashemzadeh, Hassan Dariushnejad,
Volume 22, Issue 2 (8-2020)
Abstract
Cancer is a major and serious problem for human health. Despite the many advances in the field of treatment, it remains the biggest global medical challenge. The main barrier to treating this disease is a process called metastasis, which occurs in 90% of cancers.
According to World Health Statistics, breast cancer is among the three world's prevalent cancers and the second largest cause of cancer deaths in the world that is about 1.67 million people. Bone, liver, lung and brain are common organs for metastatic breast cancer. Proprietary processes and various molecules play a role in metastasis to each of these organs. The target microorganisms first cause the cancer cells to be present in these organs, and then the release of specific factors from cancer cells and their interaction with the target micro-environment results in the survival of these cells and the formation of metastasis. In this review article, we try to find out the key molecules of these mechanisms that can be considered as an appropriate therapeutic target for breast cancer by studying the mechanisms involved in metastatic breast cancer to target organs.
Ayat Moradipour, Abdolrazagh Marzban, Maryam Karkhane, Hamed Esmaeil Lashgarian,
Volume 22, Issue 4 (1-2021)
Abstract
Background: The high prevalence of Helicobacter pylori (H.pylori) infection worldwide, especially in developing countries, and its role in gastric malignancies and the emergence of antibiotic resistance have led to proposing the different treatment and prevention methods against infection. HP associated GlmM gene is coding for Phosphoglucosamine mutase that considered for molecular detection of HP.
Materials and Methods: In this case-control study, blood and stool samples were obtained from studied population and the titers of IgG & IgA were measured by ELISA kits. DNA was extracted from stool samples and PCR was used to detect glmM gene. The results were analyzed by SPSS software.
Results: In initial study regarding of antibodies level in the blood serum, 86% of the subjects in the group showed antimicrobial IgA and IgG at the same time, while in the control group this amount was 59.9%. The results of PCR showed that in the case group, 20 out of 42 cases had glmM gene and in the control group there was no positive sample for this gene.
Conclusion: In this attempt, we tried to find a linkage between HP-associated virulence glmM gene and antibodies concentration through which probability of developing acute and severe state of disease in infected patients. The PCR and Ab titers associated results showed no significant relation between glmM gene of HP originated stool sample and serum concentration of IgG and IgA in patients who suffered from HP infection.
Ayat Moradipour, Hassan Dariushnejad, Changiz Ahmadizadeh, Hamed Esmaeil Lashgarian,
Volume 25, Issue 3 (11-2023)
Abstract
Background: Carvacrol is a natural monoterpene phenolic compound that has biological activities with therapeutic applications, and this study aimed to investigate the apoptotic effect of Carvacrol on the human breast cancer cell line MDA-MB-231.
Materials and Methods: After the preparation and cultivation of MDA-MB-231 cells, the IC50 value of Carvacrol on the cells was evaluated by the MTT assay method, and then the induction of apoptosis in the cell line treated with different concentrations of Carvacrol was observed by DAPI staining. The qPCR method was used to check the expression levels of Bax, P53, and Bcl-2 genes at the mRNA level. The results were analyzed using GraphPad Prism 9 software.
Results: The results showed that the cytotoxicity of Carvacrol against MDA-MB-231 cancer cells was dose-dependent at 24 (P=0.034) and 48 (P=0.041) hours. The IC50 of Carvacrol at 48 h was 154.2 μM. The MDA-MB-231 cells treated with Carvacrol showed induction of apoptosis from the mitochondrial pathway by increasing the expression of P53 and BAX genes and decreasing the expression of the anti-apoptotic Bcl-2 gene. In addition, the number of apoptotic cells with dense DNA increased significantly in the Carvacrol treatment group (P=0.001).
Conclusion: This study showed that treatment of the MDA-MB-231 cell line with Carvacrol can inhibit its growth and proliferation through the induction of apoptosis from the BAX/BCL-2 pathway. Considering the significant inhibitory effect of the treatment of cells with the Carvacrol compound, it seems that there is a suitable research field for using this compound in the control and treatment of breast cancer.
