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Showing 3 results for Ismaili
Study of germination indices and characters and seed establishment of myrtus communis L.
Ahmad Ismaili , Hamid Reza Eisvand , Abdollhosein Rezaeinejad , Kamran Sameey , Seyed Mahmod Zabeti ,
Volume 14, Issue 2 (7-2012)
Abstract
Background : Myrtle is a shrub and has a many applications in medicinal industries. Although the myrtle can reproduce by cutting, its reproduction by seed will bring more advantages such as economic advantages and better regeneration in nature or agriculture. Regarding to the difficult seed germination of this plant and also the need for extension the cultivation, this study was conducted for determination of germination inhibitor factors. Materials and Methods: Mechanical scarifying (by sand paper) and chemical scarifying (by immerging the seeds in 98% sulfuric acid for 2 and 4 minutes and then 3 times rising in distilled water) were used against hardseeness (physical dormancy). Cold treatments (1 and 2 months) were used for physiological dormancy. All mentioned experiments were conducted based on randomized complete design with 3 replications. Results: Study of healthy seeds showed the existence of a dual dormancy including mainly hardseedness (76%) and physiological dormancy in a part. Percent of seedling establishment was higher in mechanical treatment in compare of chemical ones and acid treatments also had some adverse effects on seedling establishment and increased the abnormality (especially on roots and vigor). Conclusion: Separating the blank seeds and mechanical scarifying are the suggesting treatments to produce high seed germination and good establishment of seedling.
Isolation and identification of probiotic lactic acid bacteria from donkey,s milk
Mohamad Javad Akrami, Farhad Nazarian Firouzabadi, Ahmad Ismaili,
Volume 17, Issue 4 (2-2016)
Abstract

Background : The utilization of donkey’s milk is increasing with regard to nutritional values and non-allergenic proteins. Donkey’s milk microbiota, especially lactic acid bacteria (LAB) has not yet been fully identified. This study was conducted to identify and isolate LAB with probiotic properties.

Materials and Methods: Specific culture-dependent techniques and standard microbiology methods were used to identify LAB. 250 isolates were randomly picked from different donkey’s milk samples different farms in Alashtar, north of Lorestan province, Iran. Gram-staining, catalase reaction, motility test, growth at different temperatures, pH 9.6 and in presence of 6.5% NaCl as well as the ability to ferment different sugars, sodium hippurate hydrolysis and light microscope analysis were investigated.

Results: Out of 250 isolates, 20 isolates were gram-positive, catalase-negative and cocci form. Enterococcus and Streptoccus genera were dominant LAB in donkey’s milk. Entreococcus faecalis (55%) and Streptococcus devriesei (45%) were among the predominant species. The results of this experiment, for the first time, reported the occurrence of Streptococcus devriesei in donkey’s milk in Iran.

Conclusion: Despite high lysozyme concentration, donkey’s milk rich in probiotic LAB can be used to feed infants with allergy to cow’s milk proteins. Both Entreococcus and Streptococcus bacteria produce different types of bacteriocins which are effective against a wide range of different type of infections. Occurrence of Streptococcus devriesei in donkey,s milk may provide new research field in Iran.


Production of a recombinant antimicrobial Dermaseptine B1 peptide in Nicotiana tabacum L. hairy roots with antibacterial activity
Ziba Nazari, Farhad Nazarian Firouzabadi, Ahmad Ismaili, Mostafa Darvishnia,
Volume 20, Issue 2 (6-2018)
Abstract
Background: Root hair culture is a valuable system to produce recombinant proteins in planta. Antimicrobial peptides (AMPs) are  vital parts of the innate immune response found in almost all forms of life. Precise target activity and limited toxicity towards mammalian cells make them suitable candidate molecules to combat evolving drug-resistant microorganisms. The aim of the present study was to produce a Dermaseptin B1 recombinant antimicrobial peptide in Nicotiana tabacum root hair and assess the antibacterial activity of the protein extract from transgenic root hairs.
Materials and Methods: A Dermaseptin B1 encoding gene sequence was C-terminally fused to a Chitin Binding Domain (CBD) encoding sequence and cloned in a plant binary vector used for Agrobacterium rhizogenes-mediated transformation to generate root hairs in tobacco. Transgenic root hairs were produced, and protein extracts were used to assess antimicrobial activity against a number of microbes.
Results: PCR and RT-PCR analysis confirmed the integration of the Dermaseptin B1 gene in a root hair cell genome and the presence of Dermaseptin B1 mRNA transcripts, respectively. Recombinant protein had a significant (P<0.05) antibacterial effect towards gram-positive and gram-negative bacteria.
Conclusion: Dermaseptine B1 recombinant peptide was successfully produced in tobacco root hair cells and its antibacterial effects was confirmed. These results suggest that the recombinant protein may have a therapeutic effect to control bacterial pathogens. It can be concluded that root hair cells can be used to produce and purify valuable recombinant proteins with pharmaceutical applications.

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