Showing 4 results for Neamati
Mirza Ali Nazarynia , Masoud Alirezaei , Mohammad Baloch , Ali Haery Rohany , Shima Neamati ,
Volume 11, Issue 5 (yafte 2010)
Abstract
Oil seeds have saturated and nonsaturated essential fatty acids that affect on the spermatogenesis process of testis. Materials and methods: In the present study, Twenty-one of male rats newly were taken from breast feeding each group ( Control, Maize oil, Peanut oil) consists of seven rats were studied. The rats were fed by maize oil and peanut oil for 10 percent (w/w) of the diet and after three months and achieving to the maturation stage with macroscopic observations, the examination of blood hormones, tissue sections of testicles, and weight and volume measurement of the testis in each group were carried out. Results: Our data of the present study showed that there was a significant decrease in the amount of testosterone and FSH in the treatment groups compared to the control group (P<0.001) while this difference about LH and volume of the testis was P<0.001 for maize oil and P<0.05 for peanut oil. However no significant decrease was observed in weight of the testicles in the treatment groups compared to the control groups. Conclusion: Taken together, the present study shows that consumption of maize oil and peanut oil can be an introduction for the synthesis of prostaglandins, due to the contain of essential fatty acids specially archidonic acid. It is known that, archidonic acid can result on the replication and proliferation of the spermatogony layer of the semenifer tubes (Spermatogenesis process) and it is enabling to affect on the basal metabolism via increase of the insulin activity in the testis. By contrast, in the tube is occurred the inhibition of spermatocyte to spermatosoide conversion.
Dr Majid Taati, Dr Masoud Alirezaei, Dr Mohammad Hadi Meshkatalsadat , Dr Bahram Rasoulian, Dr Omid Dezfolian, Shima Neamati,
Volume 13, Issue 2 (8-2011)
Abstract
It is well known that pathogenesis of ethanol in liver and kidney is directly related to increase of free radicals and oxidative stress which lead to the structural and functional damage in these two vital organs.
Materials and Methods: Fifty-six male Wistar rats were randomly divided into eight identical groups and were treated as follows: control group (normal saline), the ethanol-fed group (4 g/kg body weight), 3 groups received three doses of Ziziphus jujuba fruit extract (50, 100, 200 mg/kg body weight) and 3 groups were fed with three doses of Ziziphus jujuba fruit extract plus ethanol (50, 100, 200 mg/kg plus 4 gr /kg body weight). All treatment were applied once daily by gastric gavage for 60 consecutive days. Samples of serum, liver and renal tissues were achieved for analysis of serum components, antioxidant enzymes, and thiobarbituric acid reactive substances (TBARS) from rats.
Results: Superoxide dismutase and glutathione peroxidase (GPx) liver activities were decreased significantly in ethanol group compared to the control group. While, only glutathione peroxidase activity of liver increased significantly by administration of Z.jujuba fruit extract (200 mg/kg). The activity of kidney GPx, increased significantly in ethanol group compared to the control group. Also, kidney GPx activity decreased significantly in Z.jujuba fruit extract and ethanol group (200 mg/kg plus 4gr/kg) in comparison with the control group. In the present study, liver TBARS concentration, indicator of lipid peroxidation, increased significantly in ethanol group compared to the control group (p<0.05).
Conclusion: Pretreatment by Z.jujuba fruit extract can protect liver and kidney against ethanol–induced oxidative stress.
Masoud Alirezaei, Azadeh Khonsari , Shima Neamati , Najmeh Khalighyan, Katayon Javidnia,
Volume 14, Issue 2 (7-2012)
Abstract
Background : The histamine poisoning directly correlates to improper preservation of foods. Diamine oxidase catalyses the oxidative deamination of histamine and other biogenic amines such as cadaverine and putrescine. Plant diamine oxidases are of widespread occurrence in Leguminaceae such as Cicer arietinum, Lathyrus sativus and Vigna radiata. For this reason, this study was carried out to examine purification and enzyme kinetic properties of histaminase from the Lathyrus sativus seedling.
Materials and Methods: The purification of histaminase was according to the method given by Federico et al., with some modifications. In short, using ammonium sulfate precipitation of proteins, dialysis against potassium phosphate buffer 50mM, pH=7 over night, concentrating via dialysis bag and PEG, loading on DEAE52-sephadex column previously equilibrated with 15 mM potassium phosphate buffer pH=5.5 containing 1 µmol CuCl2 and finally enzyme eluted by increasing the NaCl concentration to 0.2 M. The homogenicity of enzyme was confirmed by SDS-PAGE in each step and diamine oxidase activity was determined by a spectrophotometer.
Results: The specific activity of the final purified enzyme was recorded at 50 U/mg protein for histamine substrate and enzyme kinetic properties of this enzyme (Km=2.5 mM, Vmax=3.05), and the homogenicity of purified enzyme by SDS-PAGE at 72 kDa region were indicated.
Conclusion: Overall, the herbal histaminase as a new enzyme in prevention of histamine poisoning is promising.
Safoura Dehghan, Ali Neamati, Pouran Ardalan,
Volume 21, Issue 2 (9-2019)
Abstract
Background: One of the important aspects of nanotechnology for use in biology is the production of nano materials with controllable size, chemical characteristics and dimensions. It can be said that the purpose of nanotechnology is to control each atom and molecule. It is now known that nanoparticles are capable of destroying cancerous cells without side effects on normal cells. Liver cancer is the fifth most common cancer in the world and the third most common cause of cancer deaths in the world. The purpose of this study was to investigate the anti-inflammatory properties of silver nanoparticles synthesized by the Amaranthus Cruentus plant on Hep G2 liver cancer cells.
Materials and Methods: The studies in this research included in vitro experiments. The anti-inflammatory effect of silver nanoparticles produced by the Amaranthus Cruentus plant was evaluated using the Real-Time PCR technique and assaying the expression of IL-1b and IL-10 genes at concentrations of 50, 100 and 200 μg/ml.
Results: The findings from the gene expression showed that the silver nanoparticles produced by the Amaranthus Cruentus plant significantly reduced anti-inflammatory activity by decreasing the expression of the IL-1b pre-inflammatory gene expression at the level of P <0.001 and increasing the expression of the anti-inflammatory IL -10 meaningful at the level of P <0.001.
Conclusion: Based on the results of this study, silver nanoparticles of the Amaranthus Cruentus plant have anti-cancer properties, and the expression of the gene leading to inflammation, IL-1b, decreased meaningfully. In addition, there was a significant increase in the expression of the IL-10 gene. Given these features, it is likely that the use of these nanoparticles, after supplementary studies, can be suggested as complementary data in the treatment of cancer and could have other biomedical applications.
