Showing 6 results for Sharafi
Shirzad Fallahi , Ebrahim Badparva , Hosein Nahrovanian , Ali Chegeni Sharafi , Farzad Ebrahimzadeh ,
Volume 9, Issue 2 (9-2007)
Abstract
Fallahi Sh1, Badparva E1, Nahrovanian H2, Chegeni Sharafi A3, Ebrahimzadeh F4 1. Instructor, Department of parasitology, Faculty of medicine, Lorestan University of medical sciences
2. PhD, Pasteur institute of Iran.
3. Master of science, parasitology
4. Instructor, Department of statistic, Faculty of health, Lorestan University of medical sciences
Abstract
Background: Intestinal parasites are the most common enteric pathogens in patients with HIV infection. These intestinal pathogens are the main cause of morbidity and mortality in HIV positive patients. There have been very few reports on the prevalence of intestinal parasites in HIV positive and AIDS patients in Iran. To investigate the prevalence of opportunistic intestinal parasites in this population, a cross-sectional study was carried out on 306 HIV positive and AIDS patients in Khorramabad city.
Materials and methods: Demographic data were collected by a questionnaire. Three stool samples were collected from every patient. Direct smear, Formalin-ether concentration techniques and Modified acid fast (Kinione) and modified trichorome staining method carried out on all samples. Data was analyzed by T-test and Chi square method.
Results: After examination’s it detect that, Prevalence of the intestinal parasite in HIV positive and AIDS patients in Khorramabad city was 22.5% and This rate was higher in AIDS patients. Moreover, we demonstrated that there is a significant relationship between age group, level of education, occupation, type of intestinal signs, variants and infection to intestinal parasites. It’s noticeable that between status of HIV/AIDS variant and infection to intestinal parasite there was a significant relationship
Conclusion: High prevalence of intestinal parasites in HIV positive and AIDS patients in Khorramabad city reflects the necessity of prevention, screening, diagnosis and treatment programs for these patients.
Mohammad Mehdi Aslani , Marjan Hahsemipour , Vajihe Sadat Nikbin , Fereshte Shahcheraghi , Akram Eidi , Zeinab Sharafi ,
Volume 11, Issue 2 (yafteh 2009)
Abstract
Background: Pseudomonas aeruginosa has emerged as one of the most important nasocomial pathogen resulting in morbidity and mortality rates.
The aim of this research was to PCR identification of P. aeruginosa isolated from tracheal samples based on the amplification of I lipoprotein (oprI) for detection of genus and L lipoprotein (oprL) for detection of species and Exotoxin A (toxA) gene.
Materials and Methods: A total of 120 P. aeruginosa clinical isolates were collected from patients with tracheal infections. Chromosomal DNA of the isolates was extracted and used for PCR of oprI, oprL and Exotoxin A (toxA).
Results: From 120 P. aeruginosa 100% were positive for oprI and oprL genes based on PCR assay and from these samples 83% were positive for ExotoxinA (toxA).
Conclusion: Using PCR method with genome and specific primers can be more accurate and sensitive than biochemical tests to identify P.aeruginosa strains. It is also replaceble method for biochemical methods.
According to the results obtained by PCR test toxA gene is not as sensitive as oprI and oprL to recognize P. aeruginosa. So using PCR of all these three genes are necessary to detecte this bacterium.
Sepideh Vahabi , Mohammad Sharafi, Fatemeh Yari,
Volume 11, Issue 3 (yafteh 2009)
Abstract
Background: Stress response to surgery and anesthesia includes wide range of hematological, immunological and endocrinological changes that partially were known. We designed this study to compare 2 methods of anesthesia, general anesthesia (GA) and spinal anesthesia (SA) at T10 level on serum cortisol and C_reactive protein (CRP) level and white blood cell (WBC) count and its differentation, as some markers of stress response. Materials and Methods: This randomized clinical trial study was carried out on 56 females in ASA class 1 undergoing elective cystocel_rectocel repair surgery in Khorramabad Asali hospital in 2004. Patients divided into 2 groups, General Anesthesia (29 patients) and Spinal Anesthesia (27patients) at level of T10. WBC count and its differentiation, serum cortisol and CRP level were measured before and 12 hs after surgery. Data analysIs was done by SPSS software. Results: Mean WBC count in GA group was significantly higher than SA group in 12 hrs post operation time (PV=0. 04l). There was no significant differences between percentages of polymorphonuclears, lymphocytes, eosinophils and monocytes in pre and post operation time in two groups(PV>0. 05). Although the mean serum cortisol level in GA group was lower in preoperative time (PV=0. 000), but in 12 hrs later was significantly higher than SA group (PV =0.0003). CRP in preoperative (PV=O. 1) and post operative (PV=0. 18) time showed no significant differences between 2 groups. Conclusion: It seems that spinal anesthesia at T10 level effectively inhibited stress response to surgery and decreased release of cortisol and WBC count in post operative time and its effect is mediated by inhibiting impulse at spinal cord level.
Behroz Farzan , Jahanbakhsh Rezanejadie , Amir Abbas Shahdost , Sepideh Vahabi , Mohammad Sharafi , Mohammad Javad Tarrahi ,
Volume 11, Issue 4 (yafte 2010)
Abstract
Background: In recent years drug abuse and addiction have crossed the health and medical borders and have been turned into a social-medical problem. Spinal anesthesia for lower extremity surgery could be used as a selective method for pulmonary and systemic diseases such as diabetes, liver and kidney disorders and so on .
Also it is possible to see pulmonary and cardiovascular diseases at the same time in addicts .In this research the rate of success in spinal anesthesia in addict and non addict males was evaluated.
Materials and Methods: This cross-sectional study was carried out on 50 patients. The patients were classified into two groups. Including 25 addicts and 25 non addicts.
The age range was selected between 25-50 years and the height range between 165-180 cm. Addiction and non addiction confirmed by morphine urine test. Spinal anesthesia was introuduced in sitting position ,the spinal needle was inserted in the L3- L4 interspace .Local anesthetic was 2cc Lidocaine 5% .
Five minutes after injection of lidocaine, the sensory blockagde was checked by needle method without patients' attention.The data were collected and finally analysed by SPSS ver 10.
Results: Spinal anesthesia was 100% successful in both addict and non addict groups. Five addicts (20%) and two non addicts (8%) felt pain during the surgery.
We evaluated sensory blockade of these patients again,all of them had not pain but malingered to have pain.
Conclusion: Spinal anesthesia is effective in addicts and is a suitable method for surgery candidates.
Omid Ali Papi , Mohammad Saki , Hamid Mokhayeri , Ardeshir Sheikh Azadi , Ali Chegeni Sharafi , Mahmod Nabavi , Roshanak Namazi , Fatemeh Azimian,
Volume 15, Issue 4 (1-2014)
Abstract
Background: Hepatitis is a disease causing inflammation of the liver and impairment of its function .It is often transmitted through contaminated blood and blood products and may become chronic presenting with flu like symptoms .Also fatigue , loss of appetite , myalgia and arthralgia , nausea and vomiting are its remarkable symptoms. The aim of this research was to study the prevalence rate of hepatitis B in rural papulation of Garab, Padervand, Chaghabal and Tang-e Panj in terms of age, sex, job… and its comparison with prevalence rate in similar regions of our province and country. Materials and Methods: Aimed to determine the seroprevalence of HbsAg infection in areas of Garab, Padervand , Chaghabal and Tang-e Panj and relationship between defined epidemiological factors and the infection in the population under study, after providing a questionnaire containing the symptoms of the disease, the data were collected and blood specimens were obtained. Then the specimens were tested by ELISA . Results : Out of a total of 454 cases 227 were male and 227 were female ( identical sex ratio ) of them 27 cases (6.2 % ) were HBsAg positive . Out of infected persons 14 were male(51.9%) and 13 were female(48.1%) . Most infected ones were 20 to 40 years old and housewives , truck drivers and students were amongst the most infected cases . Conclusion : Based on the findings of the study heptitis B infection in these areas is far more frequent than the mean national rates amounting to 2 to 3 percent that is in accordance with surveillance system data in the mentioned areas . Although the findings does not define the main transmission pattern of the disease , when age and gender distribution of infected cases is considered ( almost equal sex ratio and preponderance of married ones ) we can state that this pattern is largely directed to sexual transmission .Although all age and sex groups are at risk , men and those at the age of 20 to 40 are more prone to infection . Occupation, blood transfusion , household dimension , vaccination history were significantly related to HbsAg infection .
Rahman Soori, Firuz Sharafi Dehrahm, Sirous Choobine, Vahid Valipour Dehnou,
Volume 20, Issue 3 (10-2018)
Abstract
Background: Exercise is considered as the important treatment in diabetic patients and the process of angiogenesis in diabetes can be affected by exercise. The purpose was to investigate the effect of 6 weeks of endurance training in the form of treadmill running on VEGF levels in cardiac muscle of diabetic rats.
Materials and Methods: 24 male Wistar rats weighing 245±9.4 g and 8 weeks old were randomly assigned to control (n=6), exercise (n=6) diabetic (n=6) and diabetic and exercise (n=6). Diabetes was induced by streptozotocin (50 mg/kg). The training was performed for 6 weeks and 5 sessions per week. 24 hours after the protocol, the rats were sacrificed and their cardiac tissue extracted. The VEGF levels were measured by ELISA. To analyze data, one-way ANOVA was used.
Results: Training results showed a significant increase in VEGF in the diabetic group compared to the control group (p=0.008). The level of VEGF in the diabetic group was significantly reduced compared with the control and exercise groups (p=0.001). The level of VEGF in the diabetic and exercise group was significantly lower than the control and exercise groups (P<0.05). Exercise significantly increased VEGF in the exercise group compared to the control group (p=0.001).
Conclusion: VEGF levels were significantly higher in healthy groups than in diabetic groups and training increased the level of VEGF in the cardiac muscle, and possibly this increased levels of VEGF has positive effects on angiogenic processes in diabetic patients. Therefore, endurance training could be a valuable strategy to develop therapies for diabetes.
