Showing 15 results for ahmadvand
Mohsen Ani , Seyed Ali Asghar Moshtaqi , Hasan Ahmadvand ,
Volume 9, Issue 4 (Yafteh 2008)
Abstract
Ani M1, Moshtaqi AA1, Ahmadvand H2 1. Assistant Professor, Department of Clinical Biochemistry, Faculty of pharmacy, Isfahan University of Medical Sciences 2. Assistant Professor, Department of Clinical Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences Abstract Background: There are reports indicating that fatty acid transport is affected by many biochemical parameters including trace metals. In this study the effect of Ti on oleic acid transport is investigated Materials and methods: Male rats (200-250gr) were used for the experiments. Rats were killed, their intestines were removed and the jejunum parts were dissected. Everted Gut Sacs (EGS) were prepared from this part. Sacs were filled with buffer and incubated in a medium containing oleic acid and TiCl3. The transported oleic acid inside the EGS was measured spectrophotometrically under different condition of PH, temperature and TiCl3 concentrations. Results: Titanium decreased fatty acid uptake by entrocytes in a dose dependent manner. In a medium contained 0.5, 1, 1.5, 10 mmol of TiCl3, uptake of oleic acid was inhibited by 14.6%, 31.6%, 38.6% and 54.5% respectively. This inhibition was achieved when sodium ion (Na) was present. In the absence of sodium, uptake of oleic acid was inhibited by 3.5%, 28%, 29%, and 39% respectively which was not very significant. Conclusion: Oleic acid transport appeared to be an Na dependent process and Ti may exert its inhibitory effect by interfering with this system.
Ahmad Tamjidipur , Majid Tavafi , Hasan Ahmadvand, Alireza Khalatbari ,
Volume 11, Issue 4 (yafte 2010)
Abstract
Menopause and decrease of estrogenic hormones lead to osteoporosis. Treatment of osteoporosis by estrogenic hormones like estrogen in women has resulted in secondary complications such as cancers of breast and genital system. Activity of osteoclasts for removing bone is necessary to secretion of H+ ions on bone matrix. Today, using proton pump inhibitors to inhibit the activity of osteoclasts is under study. In other hand, recent researches show that these inhibitors by decrease absorbing of calcium intestine, can increase the risk of fracture. According to physiology of osteoclasts and the vitamin D mechanism on absorbing calcium from intestine, the combined effect of vitamin D3 and omeprazole were studied. Materials and Methods: 64 female Sprague dawlley rats (160 to 180 gr ) were selected and divided into 8 groups ( 8 in each ): 1- Sham group, without ovariectomy, 2- ovariectomized group without treatment, 3- ovariectomized group, treated with vitamin D3 100 µg ∕ kg, 4- ovariectomized group, , treated with vitamin D3 400 µg ∕ kg, 5- ovariectomized group, treated with omeprazole 20 mg/ kg and vitamin D3 100 µg/kg, 6- ovariectomized group, treated with omeprazole 20 mg/ kg and vitamin D3 400 µg/kg, 7- ovariectomized group, treated with omeprazole 20 mg/ kg, 8- ovariectomized group, treated with DMSO as a solvent. A week after ovariectomy, treatment of 3th to 8th groups began and continued for 12 weeks. During the sampling, under general anesthesia, blood were sampled and serum was prepared, and then lumbar vertebrae, femur bones and the right tibia separated and were put in the fixative solution. Serum alkaline phosphatase and Ca were measured. Resistance of femur bones were measured against fracture. Histological section prepared from L3 vertebrae and the end of tibia bones. The volume density of trabeculae of the L3 vertebrae body of samples was measured by stereologic rules. The number of osteoclasts in tibias sections was measured morphometrically, and the density of L4 vertebrae was determined based on Archimedes principle . The mean of variables was compared by SPSS 12 software. To compare the mean of alkaline phosphotase, serum Ca, the density of L4 vertebrae and the resistance of femur bones against fracture, we used ANOVA and Games-Howells test, and to compare the mean of volume density of trabeculae and the number of osteoclasts, Mann- Whitney test in p<0. 05 was used. Results: After 12 weeks, ovariectomy made severe osteoporosis in the 2nd and 7th groups . In the 1st, 3rh, 4th, 6th and 8th, no osteoporosis was delected, but in the 5th group, osteoporosis was detected slightly. Conclusion: Vitamin D treatment and almost DMSO prevent osteoporosis progressing. omeprazole treatment alone can prevent osteoporosis but in combination with a suitable dose of vitamin D, it can prevent osteoporosis and its progress.
Hasan Ahmadvand , Ali Khosrowbeigi , Shahrokh Bagheri , Reza Haji Hosseini , Farshid Ghazanfari , Leila Nemati ,
Volume 11, Issue 4 (yafte 2010)
Abstract
Oxidation of low-density lipoprotein (LDL) has been strongly suggested as a key factor in the pathogenesis of atherosclerosis. Thus the inclusion of some anti-oxidant compounds such as Satureja Khozistanica,vitamin E and coenzyme Q10 in daily dietary food stuff may inhibit the production of oxidized LDL and may decrease both the development and the progression of atherosclerosis. The present study investigated the inhibitory effects of Satureja Khozistanica, vitamin E and coenzyme Q10 on LDL peroxidation induced by CuSO4 quantitatively in vitro. Materials and Methods: LDL was incubated with CuSO4 and the formation of conjugated dienes and thiobarbituric acid reactive substances (TBARS) of LDL were monitored as markers of LDL oxidation. Inhibition of this Cu-induced oxidation was studied in the presence of extracts of Satureja Khozistanica,vitamin E and coenzyme Q10. Results: It was demonstrated that Satureja Khozistanica like vitamin E and coenzyme Q10 is able to inhibit LDL oxidation and decrease the resistance of LDL against oxidation in vitro. Conclusion: This study showed that Satureja Khozistanica similar to vitamin E and coenzyme Q10 prevented the oxidation of LDL in vitro and it may suggest that they have the similar effect in vivo
Dr Majid Tavafi, Dr Hasan Ahmadvand, Ahmad Tamjidipoor, Dr Alireza Khalatbari, Dr Bahram Delfan, Mehdi Birjandi,
Volume 12, Issue 3 (3-2011)
Abstract
Diabetic nephropathy is the common cause of leading to end stage of renal disease.Satureja Khozestanica essence for the first time used as antioxidant, antidiabetic and anti-inflammatory agent for inhibition of diabetic nephropathy. Materials and Methods: 40 male rats were uninephrectomized from left flank .The rats were divided into four groups randomly(ten per each group), group one as control, group two diabetic without treatment and groups three and four, treatment with Satureja essence with 250 ppm or 500 ppm in drinking water respectively. Diabetes was induced in the second, third and fourth groups by alloxan injection subcutaneously .After 8 weeks treatment of serum malondialdehyde was measured. Kidney paraffin sections were prepared and stained by Periodic Acid Schiff method.Glomerular volume ,intraglomerular mesangial volume and volume of glomerular capillary were estimated by stereological rules. Data were compared by SPSS13 software and Mann Whitney nonparametric test at p<0.05. Results: Glomerular hypertrophy, mesangial expansion and reduce of glomerular capillary volume in the groups that treated by Satureja essence, significantly were inhibited in comparisor with diabetic untreated group but it cannot be saved at level of control group significantly(p<0.05). The level of serum malondialdehyde in treated groups was maintained at the level of control group significantly (p<0.05). Conclusion: Satureja Khozestanica essence significantly can inhibit glomerular hypertrophy, mesangial expansion and save glomerullar capillary volume in diabetic rats significantly.
Dr Hasan Ahmadvand, Dr Majid Tavafi, Dr Gholamreza Shahsavari, Dr Ali Khosrowbeygi, Foad Abdolahpour , Shahrokh Bagheri, Leila Nemati,
Volume 13, Issue 1 (6-2011)
Abstract
The prevalence of diabets in the world is rapidly rising. Currently over 180 million persons worldwide have diabets and this number is expected to be doubled in the year 2030. In diabets stress oxidative markrs are increased. Coenzyme Q10 as a potent antioxidant can decrease lipid peroxidation and deplete free radicals. The persent study investigated the effects of coenzyme Q10 on serum lipid profile in male Wistar rats diabetics quantitatively.
Material and Methods: Twenty one male rats (200-250 gr) were randomly used in tree groups for the experiments(7 rats per group). First group as control, second group as untreated diabetic and third group as treated diabetic with Coenzyme Q10 (15 mg/kg). Second and third groups were diabetic with injection of alloxan(120 mg/kg). After six weeks treatment their serum lipids,were measured. Data were analyzed using SPSS software. Data were compared by Mann Whitney test.
Results: The results indicated that Coenzyme Q10 decreased serum triglyceride , cholesterole , LDL and VLDL in treated diabetic groups in comparision with untreated diabetic groups respectively, but serum HDL didn't increase(P<0.05).
Conclusion: Regarding the fact that Coenzyme Q10 decreases serum lipids. Our data suggest that Coenzyme Q10 has beneficial effects on diabetes induced complications.
Poran Toolabi, Majid Tavafi , Soheila Ebrahimi, Sima Nasri, Hasan Ahmadvand ,
Volume 13, Issue 3 (12-2011)
Abstract
Background : Administration of Gentamicin is restricted due to its nephrotoxicity. Many studies have demonstrated that free radicals induced Gentamicin- nephrotoxicity. Olive leaf contains some antioxidant biophenols such as Oleuropin. In this study for the first time the effect of hydroalcohlic olive leaf extract on Gentamicin-induced nephrotoxicity was investigated.
Materials and Methods: 35 male Sprague-Dowley rates were randomly divided into five groups. The first group served as a control and injected with the normal saline. Nephrotoxicity induced by Gentamicin (100 mg/kg) in experimental groups for 12 days. Three groups were treated with hydroalcohlic olive leaf extract (25, 50 and 100 mg/kg respectively). The rats were killed on the twelfth day.Plasma Malondialdehyde (MDA) and creatinine concentrations were measured. Kidney sections were prepared and stained with PAS method for histopathological studies.
Results: hydroalcohlic olive leaf extract at 25, 50 and 100 mg/kg/d significantly reduced gentamicin-induced tubular necrosis (p<0.05) and inhibited decreases of proximal tubule volume density (p<0.05). hydroalcohlic olive leaf extract at 50 and 100 mg/kg/d significantly reduced lymphocyte infiltration in comparison with Gentamicin administrated rats (p<0.05). Gentamicin increased level of MDA. Treatment with extract (100 mg/kg/d), significantly reduced level of MDA compared with other groups (p<0.05).Use of extract at 25, 50 and 100 mg/kg/d significantly inhibited increase of serum creatinine (p<0.001). Treatment with extract (50 mg/kg/d), significantly inhibited increase of serum urea (p<0.05).
Conclusion: Hydroaleohlic olive leaf extract can reduce Gentamicin-induced nephrotoxicity and this effect is probably depends on antioxidant properties of this extract.
Hasan Ahmadvand, Hamze Amiri, Saeede Ekbatan Hamadani , Shahrokh Bagheri ,
Volume 14, Issue 2 (7-2012)
Abstract
Background : Antioxidants are compounds that protect the body against damage caused by oxidative stress that induce by free radicals. The aim of present work is to compare the various antioxidative activities of leaves hydroalcholic extract and essential oil of Vitex pseudo-negundo.
Materials and Methods: In this experimental study, leaves hydroalcholic extract and essential oil of Vitex pseudo-negundo were prepared and then radical scavenging activity of samples was assessed by using Diphenylpicrylhydrazyl (DPPH). Total antioxidant capacity samples was assessed by method of phosphomolybdat. The amount of total phenol and flavonoid samples was assessed by Folin-Ciocalteu and Zhishen methods
Results: The results showed that total antioxidant capacity Leaves hydroalcholic extract and essential oil of Vitex pseudo-negundo is (1.76±0.25 2.12±0.60), phenol content (133.11±3.00 22.00±2.00), and flavonoid content (18.00±1.00 5.50±4.00). In the DPPH scavenging assay, the IC50 (the concentration required to scavenge 50% of radical) values of leaves hydroalcholic extract and essential oil and Butylated hydroxytoluene (BHT) as refernce were (224.75±4.52 133.00±3 3.88±1μg/ml), respectively.
Conclusion: This study showed that Vitex pseudo-negundo extract is an source easily accessible of natural antioxidants and it may be suitable for use in food and pharmaceutical applications.
Ali Khosrowbeygi , Hasan Ahmadvand ,
Volume 14, Issue 3 (9-2012)
Abstract
Background : Preeclampsia is a major cause of maternal and perinatal morbidity and mortality. The aim of the present study was to assess serum levels of isoprostane F2α, malondialdehyde and total homocysteine in preeclamptic women compared with normal pregnant women.
Materials and Methods: A cross-sectional study was designed. The patients consisted of 30 preeclamptic women. The normal group consisted of 30 healthy pregnant women. Serum level of malondialdehyde was measured by colorimetric method. Isoprostane F2α and homocysteine levels were assessed using commercially available enzyme-linked immunosorbent assay (ELISA) methods. The Mann-Whitney U test was used for statistical analysis. A p value less than 0.05 was considered significant.
Results: Serum levels of isoprostane F2α, malondialdehyde and homocysteine were significantly higher in preeclamptic group than in normal pregnant women (p < 0.05). All of the biomarkers were significantly higher in women with severe preeclampsia than in mild preeclamptic women (p < 0.05).
Conclusion: The findings of the present study suggest that lipid peroxidation caused by oxidative stress might be involved in the pathogenesis of preeclampsia.
Hasan Ahmadvand , Farshid Ghazanfari , Reza Yarahmadi , Mona Mankavi , Rezvan Vajdian,
Volume 15, Issue 2 (8-2013)
Abstract
Background : Oxidation of lipids such as low-density lipoprotein (LDL) has been strongly suggested as a key factor in the pathogenesis of atherosclerosis. Thus the inclusion of some anti-oxidant compounds such as vitamin E and Satureja Khozistanica Essential oil (SKE) in daily dietary food stuff may inhibit the production of oxidized LDL and may decrease both the development and the progression of atherosclerosis. The aim of this research is to study inhibitory effects of different concentrations of essential oil Satureja Khozistanica on LDL oxidation in vitro.
Materials and Methods:Fasting blood samples from normal people after an overnight fasting were collected and then isolated LDL was incubated without CuSO4 as control and incubated with CuSO4 and several concentration of SKE (50, 100 and 200 mg/ml) and vitamin E as positive control (100 µM). Then the formation of conjugated dienes, lag time and thiobarbituric acid reactive substances (TBARS)were measured. Inhibition of this Cu-induced oxidation was studied in the presence of several concentration of SKE and vitamin E.
Results: The results showed that SKE induced LDL oxidation and decrease the resistance of LDL against oxidation in vitro. SKE at concentrations of 50, 100 and 2000 µg/ml, and vitamin E (100 µM) showed an increase rate of 33.33, 66.66, 100.00 and 111.11% respectively, against oxidation in vitro. The inhibitory effects of the SKE on LDL oxidation were dose-dependent at concentrations of 50, 100 and 200 µg/ml.
Conclusion: This study showed that Satureja Khozistanica prevented the oxidation of LDL in vitro and it may suggest that it has the similar effects in vivo.
Saeed Nazeri , Mehdi Hedayati , Hasan Ahmadvand ,
Volume 15, Issue 3 (9-2013)
Abstract
Background : Oxidative stress plays an important role in the pathogenesis of some diseases such as lung cancer, chronic obstructive pulmonary diseases and atheroscleorosis. Smoking may enhance oxidative stress through the production of reactive oxygen radicals in smoke and weakens the antioxidant defense system.The present study was conducted to determine the effect of cigarette smoking on superoxide dismutase, catalase enzymes activities and total antioxidant capacity in cigarette smokers.
Methods: This research was done on thirty male volunteers (25-35 years-old) who smoked over 10 cigarettes per day. Controls were thirty non-smoker male volunteers (25-35 years-old). Blood samples from overnights fasted subjects were used for the study. Catalase and superoxide dismutase activity and total anti oxidation capacity were analyzed. Data was described as mean±SD and data means of the two groups was compared by independent T-test. Data was analyzed by SPSS 18 software.
Results: The amount of total antioxidant capacity and catalase and superoxide dismutase activity had a significant statistical decrease in the group compared to the control group (p<0.05).
Conclusion: This study showed that oxidative stress causes a decline of the body antioxidant protective system which in long time it can cause cardiac and blood-vessels diseases in cigarette smokers.
Hasan Ahmadvand , Ali Khosrowbeygi , Gholamreza Shahsavari , Maryam Hormozi , Foad Abdolahpour , Shahrokh Bagheri ,
Volume 16, Issue 4 (2-2015)
Abstract
Background: The prevalence of diabetes is rising in the world. The aim of this study was to determine the possible protective effect of sodium selenite on serum lipid profile and atherogenic indexes in Alloxan-induced Type 1 diabetic rats. Materials and Methods: Forty Sprage-dawley male rats were divided into four groups randomly group one, as control, group two, sham (control treated with sodium selenite (1mg/kg)), group three, diabetic untreated, and group four, treated with sodium selenite (1mg/kg), respectively. Diabetes was induced in the second and third groups by alloxan injection subcutaneously. After 8 weeks, animals were anaesthetized, blood samples were also collected before killing to measure the blood glucose, triglyceride, cholesterol, LDL, VLDL, HDL and serum atherogenic indexes. Results: Sdium selenite inhibited a significant decrease of BG, TG, C, LDL and VLDL. HDL level. Sodium selenite inhibited significantly serum atherogenic indexes level. Conclusion: This study showed that sodium selenite exerts beneficial effects on the BG, lipid profile and atherogenic indexes in alloxan-induced Type 1 diabetic rats.
Saeid Veiskarami, Mohamad Fathi, Hasan Ahmadvand, Ladan Rashidi, Reza Mohammad Rezaei Khorramabadi ,
Volume 18, Issue 4 (3-2017)
Abstract
Background: free radicals produced as a result of heavy training exercise especially oxygen species (ROS) damage to body tissues Which can be prevent from this by consuming antioxidant substances timely. The aim of this study was to evaluate effect of Pomegranate (Punica Granatum) peel hydro alcoholic on reduced of oxidative stress induced by an exhaustive exercise.
Materials and Methods: Thirty two weight-matched male Wistar rats were evenly divided into: 1) control: received saline (0.2 ml saline/ rat) by oral administration via epigastric tube. 2) Received oral administration of 200 mg/kg pomegranate peel hydro alcoholic extract (PPHE200). 3) Received oral administration of 250 mg/kg Pomegranate peel hydro alcoholic extract (PPHE250). 4) Received oral administration of vitamin E (vit E) 5 mg/kg. Animals were submitted to swimming exhaustive exercise stress for an 8-week. At the end of the experiment, blood samples were collected for serum. Serum samples were analyzed for paraoxonase-1(PON-1) and myeloperoxidase (MPO) activities and nitric oxide levels.
Results: Paraoxonase-1 (PON-1) activities serum were significantly increases in PPHE200 (23.03±1.47), PPHE250 (23.59±1.98) and vit E (25.38±2.65) than in the control (18.57±1.380) (p<0.05).In PPHE200 (32.76±9.97) ،PPHE250 (31.45±6.05) and vit E (24.94±4.65) treated animals was determined in serum where myeloperoxidase activities reduced significantly compared with control (40.70±6.14) (p<0.05). Levels of Nitric oxide levels were significantly lower in PPHE 200 (46.59±2.48), PPHE250 (40.27±2.62) and vit E (36.25±3.82) treated than in control (47.18±5.36) (p<0.05).
Conclusion: Results indicated that Pomegranate peel hydro alcoholic extract supplementations can strength antioxidant defense system and anti-inflammatory induced by exhaustive exercise.
Farid Bahrami, Mohamad Fathi, Hasan Ahmadvand, Naser Pajohi,
Volume 20, Issue 1 (4-2018)
Abstract
Background : Skeletal muscles are composed of various contracted fibrils, which are mainly divided into fast-twitch and slow-twitch. This study aimed to investigate 8 weeks endurance activity on the MEF2 and HDACA4 gene expression in fast-twitch and slow-twitch skeletal muscles in male Wistar rats.
Materials and Methods: in order to carry out this study, 20 heads of male Wistar rats, age 4 weeks (110± 10), were bought from the Razi Institute of Lorestan Medical University. The same laboratory conditions were provided for the rats for the completion of 14 days of an endurance familiarization course to teach running on treadmill. At the end of this course, the rats were randomly divided into 2 groups. Experimental group (n=10 head) and control group (n= 10 head). An eight week endurance program, 5 sessions per week, was performed for the experimental group.
Results: this study showed that there was no significant change in the relative gene expression of HDACA4 and MEF2 in EDL muscle in either group (P>0.05). However, the relative gene expression of MEF2 in the experimental group was not statically significant in comparison to the control group (P>0.05). In sol muscles, there was no statically significant changes in either group’s gene expression. The relative gene expression of MEF2 in the experimental group showed a statistically significant reduction in comparison to the control group (P>0.05).
Conclusion: in summary, the results of this research have shown that doing 8 weeks endurance exercises did not cause any changes in HDAC4 and MEF2 gene expression in EDL muscle. Although in the SOL muscle, MEF2 gene expression decreased, no changes in the level of HDAC4 gene expression were observed.
Sajad Papi, Hassan Ahmadvand, Fattah Sotoodehnejadnematalahi, Dr. Parichehreh Yaghmaei,
Volume 23, Issue 0 (11-2021)
Abstract
Background: Renal ischemia-reperfusion can lead to acute renal failure. The use of antioxidants is a good way to reduce the damage caused by renal ischemia-reperfusion. Therefore, the aim of this study was to investigate the protective effect of indole-3-acetic acid on renal ischemia-reperfusion injury through its antioxidant properties in a rat model.
Materials and Methods: Forty male rats were treated for 2 weeks in four groups included (control group, ischemia-reperfusion control group, ischemia-reperfusion group with 40 mg/kg indole acetic acid treatment and ischemia-reperfusion group with 60 mg/kg indole acetic acid treatment). Then, the blood sample was taken from the heart. Serum urea and creatinine levels were measured using biochemical kits and superoxide dismutase enzyme levels were measured using photometric methods.
Results: The results showed that ischemia-reperfusion caused a significant increase in urea )P≤0/05) and creatinine) P≤0/05) levels and a significant decrease in the level of superoxide dismutase in ischemia-reperfusion rats compared to the control group. However, indole acetic acid caused a significant decrease in serum urea) P≤0/05) and creatinine e)P≤0/05) levels and a non-significant increase in superoxide dismutase enzyme compared to the ischemia-reperfusion control group )P>0/05).
Conclusion: The use of indol acetic acid was involved in reducing the renal biochemical parameters of urea and creatinine, which were increased due to ischemia-reperfusion injury, and caused the level of Super oxide dismutase enzyme to increase non-significant.
Mandana Beigi Boroujeni, Atena Salehi Marzijerani, Nasim Beigi Boroujeni, Hassan Ahmadvand, Maryam Hormozi,
Volume 25, Issue 2 (8-2023)
Abstract
Background: There is much evidence-based research on the anti-cancerous effect of antioxidants. Dihydroxyphenylethanol is a potent antioxidant that has several activities, such as oxidant stress control, cell proliferation inhibitory activity, and apoptosis induction. The present study aimed to evaluate the effect of dihydroxyphenylethanol on antioxidant enzyme activity and malondialdehyde level in the HCT-116 cell line.
Materials and Methods: In this study, a human colorectal cancer cell line HCT-116 was treated with different concentrations of 3, 4-dihydroxyphenylethanol (50, 100, 150, and 200 μM) for 24 h. Then, the level of malondialdehyde and activity of enzymes of catalase, superoxide dismutase, and glutathione peroxidase were measured by colorimetric methods.
Results: The results showed that 3,4-dihydroxyphenylethanol administration resulted in significant reduction of the malondialdehyde concentration and also significant increase of the antioxidant enzymes (e.g., catalase, superoxide dismutase, and glutathione peroxidase) in the treatment groups compared to the control group (P<0.05).
Conclusion: Overall, 3, 4-dihydroxyphenylethanol may result in reduction of oxidative stress on HCT-116 line through changes in concentration of the malondialdehyde and antioxidant enzymes activity.
