Showing 6 results for Rats
Majid Tavafi , Abdolahman Dezfulian, Mohammad Hadi Kochek , Hayat Mombeini , Asadollah Tavakoli , Mohammad Javad Tarahi ,
Volume 6, Issue 4 (2-2005)
Abstract
Background: Diabetic nephropathy is one of the cause of the end stage of renal disease (ESRD). Hyperglicemia activate intrarenal Renin-Angiotensin system and highly AngiotensinII is produced locally in glomeruli. In this research AT1(Angiotensin type 1) receptors blocked by Losartan and effects of this drug in inhibition of renal structural lesions were assessed quantitatively.
Materials & methods: 24 male rats (2 month ages) were uninephrectomised from left flank and then randomly divided in 3 groups (8 per group), then Diabetes were induced in second and third groups by injection of alloxan tetrahydrate (120 mg/kg) subcutaneously. Five days after diabetes induction third group received losartan (5 mg/kg/day) orally for 8 weeks. After 8 weeks kidneys from all groups were sampled, fixed and each kidney sliced in 1 mm thickness (systematic random sampling) for stereological study. After tissue processing section pairs were made with 5 micron thickness from each slice and stained by PAS method. Kidney volume, glomerular volume and glomerular number estimated by cavalieri and physical disector methods.
Results: Significancy test between means variables in groups were analyzed with Mann Withney statistical test in P<0.05 by SPSS12 software. For three variables (kidney volume,glomerular volume and glomerular number) losartan treated group showed significant difference to in comparison nontreated diabetic group.
Conclusion: Chronic low dose usage of losartan in uninephrectomized diabetic rats can prevent kidney volume increasing (84%), inhibit increase glomerular volume (67%) and prevent glomerular number decreasing (68%) significantly. Combined drug therapy is recommended for better results.
Abdolrahman Haj-Dezfulian , Majid Tavafi , Asadollah Tavakoli , Mohammad Javad Tarahi ,
Volume 8, Issue 2 (1-2007)
Abstract
Background: Diabetic nephropathy is one of the causes of end stage renal diseases (ESRD). Increase of IGF-1(insulin like growth factor) and GH (growth hormone) in diabetes induce kidney lesions especially Intraglomerular mesangial expansion, glomerular sclerosis and finally nephron dysfunction. In this research, IGF-1 and GH production inhibition by octreotide and sclerosis inhibition assessed quantitatively.
Materials and methods: 21 male rats (2-month ages) were uninephrectomized from left flank and then randomly divided in 3 groups (7 per group). Diabetes was induced in second and third groups by injection of alloxan tetrahydrate (120 mg/kg) subcutaneously. Five days after diabetes induction, third group received octreotide (10 μg/day) subcutaneously for 8 weeks. After 8 weeks, kidneys from all groups were removed, fixed and sliced in 1 mm thickness for stereological study. After tissue processing, sections with 5 micron thickness were prepared from each slice and stained by PAS method. Cortex volume, glomerular volume and glomerular mesangium volume were estimated by Cavalieri and point counting methods. Mean difference of variables was analyzed by Mann-Whitney statistical test at P<0.05 level using SPSS V.12.0.
Results: Chronic low dose usage of octreotide in diabetic rats can prevent increase of cortex volume (67%) and glomerular mesangial expansion (53%) significantly. This treatment inhibits increase of glomerular volume by 26% which is not significant.
Conclusion: Inhibition of cortex volume and mesangial expansion in octreotide treated group showed significant effects in comparison to the non-treated diabetic group. But this treatment has no significant effect on glomerular hypertrophy in diabetic rats.
Gholamreza Shahsavari , Abdolvahab Ehsani- Zonouz , Masoud Houshmand , Ghasem Ahangari , Mohsen Firoozrai ,
Volume 10, Issue 4 (3-2009)
Abstract
Background: The effect of the wild SKEO on activities and genes expression of hepatic glucokinase (GK), glycogen phosphorylase (GP) and phosphoenolpyruvate carboxykinase (PEPCK) in normal and diabetic rats was evaluated. Materials and Methods: The wild SKEO was orally administered at dose (100 mg/kg per day) to normal, as well as diabetic rats for 21 days. The levels of mRNA were determined using the quantitative real- time RT-PCR technique. Results: The plasma glucose concentrations of diabetic rats receiving the wild SKEO compared with diabetic control were significantly decreased. Hepatic GK and GP activities and their mRNA levels of diabetic rats treated with the wild SKEO moderately increased compared with diabetic control.. The activity of hepatic PEPCK and its mRNA levels were significantly decreased in normal rats treated the wild SKEO . The enhancement of PEPCK activity and its mRNA levels of diabetic treated rats with was significantly decreased compared with diabetic control (P<0.05). Conclusion: Our results indicate that a moderate enhancement of GP and GK as well as an excessive inhibition of PEPCK in liver of STZ-induced diabetic rats treated with the wild SKEO may contribute to the plasma glucose lowering action of SKEO that seems to be in relation with antioxidant properties of SKEO.
Leila Zarei, Mehri Kouhkan, Rahim Mohammadi, Amir Bolouri,
Volume 22, Issue 1 (4-2020)
Abstract
Background: Wound healing is the interaction of a complex cascade of cellular and biochemical actions leading to the restoration of structural and functional integrity with regaining the strength of injured tissues. Diabetes is effective in delaying the wound healing process in human beings. It has been reported that pyrozilidine derivatives bear antioxidant activity and antioxidant agents that accelerate wound healing process. The aim of the present study was to assess the activity of Methyl 2'-Methyl-1,3-dioxo-1,1',2',3,5',6',7',7a'-octahydrospiro[indene-2,3'-pyrrolizidine]-2' carboxylate (6) on the full-thickness excisional wound healing in diabetic rats.
Material and Methods: 60 male Wistar rats were randomized into three groups of 20 animals following the creation of the wound. Group 1 (normal control): creation of the wound with no further intervention. Group 2 (diabetic control): induction of diabetes in the animals and creation of the wound with the intraperitoneal administration of 100 microliter DMSO (0.25%) for one week. Group 3 (treatment): induction of diabetes in the animals and creation of the wound with the intraperitoneal administration of 100 microliter of the synthetized agent (60 µg/kg) dissolved in DMSO (0.25%) for one week. Induction of diabetes in the animals of groups 2 and 3 was performed using streptozotocin. Histologic studies were conducted on the days 7, 14 and 21 post wounding. Planimetric studies were carried out on the days 3, 6, 9, 12, 15, 18 and 21 post wounding.
Results: The histologic studies indicated a significant decrease in inflammatory cells and a noticeable increase in fibroblasts (P<0.05).
Conclusion: It could be concluded that treatment with the synthetized agent (6) could increase wound healing rate in diabetic rats.
Farhad Fathipur, Leila Zarei, Rahim Mohammadi,
Volume 23, Issue 0 (11-2021)
Abstract
Background: The aim of this study was to perform a functional and histological study of the effect of coenzyme Q10 using allografts in a rat sciatic nerve repair model.
Materials and Methods: 45 male rats were randomly divided into three groups of 15. The first group received 10 μl of sterile normal saline buffer solution intraperitoneally for one week. Buffer was injected intraperitoneally to each rat. In the third group, the left sciatic nerve was removed and each rat received the sciatic nerve extracted from the second group and was injected intraperitoneally with the same volume of coenzyme Q10 at a dose of 200 mg / kg / day for one week. Rats underwent histological evaluation and histomorphometric studies and sciatic nerve function index at 4, 8 and 12 weeks postoperatively.
Results: The morphometric and functional indices of the sciatic nerve in the third group were significantly different from the second group (P <0.05). These indicators were improved in the third group.
Conclusion: The results of this study showed that topical injection of coenzyme Q10 in the inflammatory phase after rat sciatic nerve resection accelerated the process of nerve regeneration by reducing the process of secondary damage.
Ehsan Saboory, Mones Moloody, Azar Pad, Leila Zarei,
Volume 24, Issue 4 (3-2023)
Abstract
Background: Peripheral nerve injuries are among the serious health problems of today's societies. These injuries cause long-term disabilities that last until the end of life. This study aimed to histologically and functionally determine the effect of alpha-tocopherol using allografts in a rat sciatic nerve repair model.
Materials and Methods: In this experimental study, 45 male rats were divided into three groups (n=15 each). Group 1 was designated as the healthy group without nerve manipulation. In group 2, as the positive control group, the rats received 10 μl of sterile normal saline buffer solution intraperitoneally for one week. In the third group, designated as the treatment group (allografts, alpha-tocopherol), alpha-tocopherol was intraperitoneally injected with the same volume at a dose of 300 mg/kg/day for one week. Subsequently, the rats underwent histological evaluation postoperatively at the 4th, 8th, and 12th weeks following the operation. The collected data were entered into SPSS version 17 software. After evaluating the normality of the data by the Shapiro-Wilks test, the results were reported as mean ± standard deviation, and were analised by One Way ANOVA tests and Tukey,s post HOC test under significance level of 0.05.
Results: The results showed that the morphometric and functional indices of the sciatic nerve in the third group were significantly different from the second group (P<0.05). These indicators were improved in the third group.
Conclusion: Treatment with alpha-tocopherol and allografts resulted in significant differences with the control and allografts groups in terms of axon diameter, the number of nerves, and the thickness of the myelin sheath. These differences imply the beneficial effect of this drug on inflammatory cells in the spinal cord and brain.
