Gholam Hosein Farjah , Mohammad Taghi Joghataei , Mehdi Mehdizadeh , Malihe Nobakht , Malek Sadat Naimi ,
Volume 7, Issue 3 (12-2005)
Abstract
Background: The limited availability of donor sites for nerve grafting has continued to stimulate research toward finding suitable alternatives for autograft. The aim of this research was to study the effect of piezoelectric channel and collagen gel on nerve regeneration.
Materials and methods: This research is an experimental study. Forty eight male rats (200-250 gr) were used. After axotomy, 1cm segment of the sciatic nerve in rats was resected, and the gap was then bridged by one of the four methods (Polyvinylidene fluoride or PVDF + Collagen gel, Autograft, Sham and Control). All animals were evaluated at 7, 21, 35, 46, 60 and 90 days after operation by sensory test, sciatic functional index (SFI) and at 90 days by electrophysiologic testing. Sciatic nerve was studied by light and electron microscope after 4 and 12 weeks of implantation.
Findings: At 90 days after operation, there was no difference in sensory testing between groups. The mean SFI at the 9 weeks, in autograft group was superior to that of Collagen gel + PVDF group (P<0.01). Mean motor nerve conduction velocity in the difference between experimental groups was not significant (P=0.05). After 4 weeks of implantation, regenerated tissue cables into PVDF tube were round shaped and the mean number of myelinated axons in Autograft group was superior to that of Collagen gel + PVDF group (P<0.001). Mean number of myelinated axons in Collagen gel + PVDF group were lower than autograft group (P<0.01), after 12 weeks of implantation.
Conclusion: The positive effects of collagen gel on regeneration of nerve through a PVDF channel seen in this study suggest that it may by useful for treating peripheral nerve injuries.
Ehsan Saboory, Mones Moloody, Azar Pad, Leila Zarei,
Volume 24, Issue 4 (3-2023)
Abstract
Background: Peripheral nerve injuries are among the serious health problems of today's societies. These injuries cause long-term disabilities that last until the end of life. This study aimed to histologically and functionally determine the effect of alpha-tocopherol using allografts in a rat sciatic nerve repair model.
Materials and Methods: In this experimental study, 45 male rats were divided into three groups (n=15 each). Group 1 was designated as the healthy group without nerve manipulation. In group 2, as the positive control group, the rats received 10 μl of sterile normal saline buffer solution intraperitoneally for one week. In the third group, designated as the treatment group (allografts, alpha-tocopherol), alpha-tocopherol was intraperitoneally injected with the same volume at a dose of 300 mg/kg/day for one week. Subsequently, the rats underwent histological evaluation postoperatively at the 4th, 8th, and 12th weeks following the operation. The collected data were entered into SPSS version 17 software. After evaluating the normality of the data by the Shapiro-Wilks test, the results were reported as mean ± standard deviation, and were analised by One Way ANOVA tests and Tukey,s post HOC test under significance level of 0.05.
Results: The results showed that the morphometric and functional indices of the sciatic nerve in the third group were significantly different from the second group (P<0.05). These indicators were improved in the third group.
Conclusion: Treatment with alpha-tocopherol and allografts resulted in significant differences with the control and allografts groups in terms of axon diameter, the number of nerves, and the thickness of the myelin sheath. These differences imply the beneficial effect of this drug on inflammatory cells in the spinal cord and brain.
