Volume 8, Number 2 (1-2007)                   yafte 2007, 8(2): 77-81 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shams S, Tokmehdashi H. Evaluation of the cytotoxic effects of zinc on Raji cell line by florescent technique. yafte. 2007; 8 (2) :77-81
URL: http://yafte.lums.ac.ir/article-1-1016-en.html

Abstract:   (5874 Views)
Background: Zinc has important effects on the human health, especially on the structural and functional activities of immune system. This study was carried out to examine the in vitro cytotoxic effects of Zinc on the Raji cell line. Materials and methods: The cell line was exposed to different concentrations of Zinc followed by incubation (37◦C, 5% Co2) at various time points (12 to 72 hrs). Viability and proliferation of Raji cells were then evaluated with florescent (10µl Ethidium-Bromide and Acridine-Orange) staining. Data were analyzed by SPSS (Dunnet and Variance Analysis tests). Results: There was not a significant difference in the response of the Raji cells to different amount of zinc up to 100µM at different incubation time points. At higher concentrations (200-500µM) of Zinc, viability diminished significantly at 12, 24 and more hrs of incubation time when compared to the controls (p<0.05). Conclusion: We conclude that Zn has dose-dependent cytotoxic effects on Raji cells and probably could be used for immune-modulation.
Full-Text [PDF 152 kb]   (1023 Downloads)    
Type of Study: Research |
Received: 2013/01/15

Add your comments about this article : Your username or email:
Write the security code in the box

Send email to the article author


© 2015 All Rights Reserved | Yafteh

Designed & Developed by : Yektaweb