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Professor Lorestan University
Abstract:   (249 Views)
Background: Hairy root culture is a valuable system to produce recombinant proteins in planta Antimicrobial peptides (AMPs) are a very vital parts of innate immune response found in almost all forms of life. Precise target activity and limited toxicity towards mammalian cells, make such a suitable candidates molecules to combat evolving drug-resistant microorganisms. The aim of the present study was to produce a Dermaseptin B1 recombinant antimicrobial peptide in Nicotiana tabacum hairy roots and assess the antibacterial activity of protein extract from transgenic plant.
Materials and Methods: A Dermaseptin B1 encoding gene sequence was C-terminally fused to a Chitin Binding Domain (CBD) encoding sequence and cloned in a plant binary vector used for Agrobacterium rhizogenes-mediated transformation to generate hairy roots in tobacco. Transgenic hairy roots were produced, and protein extracts were used to assess antimicrobial activity against a number of microbes.
Results: Results: PCR and RT-PCR analysis confirmed the integration of Dermaseptin B1 gene in hairy root cells genome and presence of Dermaseptin B1 mRNA transcripts, respectively. Recombinant protein had a significant (P<0.05) antibacterial effect towards gram-positive and gram-negative bacteria.
Conclusion: Dermaseptine B1 recombinant peptide was successfully produced in tobacco hairy roots and its antibacterial effects was confirmed. This Results, suggesting that the recombinant protein may have a therapeutic effect to control bacterial pathogens. It can be concluded that hairy roots can be used to produce and purify valuable recombinant proteins with pharmaceutical applications.
     
Type of Study: Research | Subject: میکروب شناسی
Received: 2018/02/13

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