Volume 23 -                   yafte 2021, 23 - : 304-318 | Back to browse issues page

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Yahyaei B, Sabbagh M. Investigation of accumulation and tissue effects due to injection of biologically produced magnetic iron nanoparticles in renal tissue of rats in response to electromagnetic field by histopathological and ICP methods. yafte. 2021; 23 :304-318
URL: http://yafte.lums.ac.ir/article-1-3327-en.html
Department of Medical Sciences, Shahrood Branch, Islamic Azad University, Shahrood, Iran
Abstract:   (333 Views)
Background: In the present study, an attempt was made to produce magnetic nanoparticles in magnetic biology and to study their cytotoxic properties in fibroblast cells and to investigate their behavior in living organisms in the presence and absence of electromagnetic fields in kidney tissue.
Materials and Methods: Magnetic iron nanoparticles were produced using Fusarium oxysporoum and iron chloride. TEM and X-ray diffraction were confirmed using spectrophotometry. Then the cytotoxicity of nanoparticles was investigated by MTT method in non-cancerous fibroblast cell culture. Finally, the IC50 nanoparticles were determined in cell culture and nanoparticles with non-toxic doses were injected into rats. Rats were divided into 4 control groups, the group receiving nanoparticles without the presence of electromagnetic field, the group receiving nanoparticles with the presence of electromagnetic field, the group without receiving nanoparticles and with the presence of electromagnetic field.
Results: Magnetic iron nanoparticles could be produced by Fusarium oxysporum. The resulting nanoparticles were about 35 nm in size and applied dose-dependent toxicity in cell culture. The biological nanoparticles did not have severe toxicity in kidney tissue and only in the presence of the electromagnetic field, slight changes in connective tissue and blood as well as changes in renal glomeruli were observed, although in the group without electromagnetic field, slight changes were observed in glomerulus.
Conclusion: Biological iron nanoparticles are non-toxic in the body and can be used in in vivo research with the aim of drug delivery to treat cancers and other diseases.
Full-Text [PDF 750 kb]   (185 Downloads)    
Type of Study: Research |
Received: 2021/05/29 | Accepted: 2021/11/11 | Published: 2021/12/20

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