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Zabihi A, Mahmoodi M. Evaluation of Diabetic Wound Healing and Collagen Changes after Subdermal Fibroblast Cells Injection in Animal Model. yafte 2021; 23 :149-160
URL: http://yafte.lums.ac.ir/article-1-3353-en.html

Background: In recent years, the number of patients suffering from diabetic ulcers has increased and many studies have shown that stem cells can be effective in healing of skin ulcers. Common diabetic wound therapies face serious failures. The aim of this study was to evaluate the healing of diabetic wounds and collagen changes following injection of fibroblast cells in male rats.
Material and Method: In this experimental study, streptozotocin induced diabetic male Wistar rats (n=16) were divided into two groups: control and foreskin derived fibroblast treated group. Wounds with a diameter of 0.8 cm were created in the back of rats and after subdermal injection fibroblast cells, the wound was evaluated by photographic method and Mason’s trichrome staining on days 7 and 21. Data were analyzed using t-test and one-way analysis of variance.
Results: The healing and collagen production rate significantly increased in fibroblast cells treated group 7 and 21 days after treatment compared with control group (P<0.01).
Conclusion: Subdermal injection of foreskin derived fibroblast cells causes more collagen production and fast healing of diabetic wound therefore can be applied in cell therapy.