Volume 14, Number 1 (6-2012)                   yafte 2012, 14(1): 39-43 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Nabiuni M, delfan B, nazari Z, Angaji A. The effects of curcumin on Aquaporin 1 level in choroidal epithelial cells of lateral ventricle in Wistar rats. yafte. 2012; 14 (1) :39-43
URL: http://yafte.lums.ac.ir/article-1-707-en.html

Department of cell and Molecular Lorestan University of Medical Sciences
Abstract:   (8644 Views)
Background : Aquaporin1 (AQP1) protoin channels that expressed in the Choroid plexuses of brain ventricles, have an important role in cerebrospinal fluid (CSF) production. Some pathophysiological conditions such as intracranial hypertension, systemic hyponatremia and hydrocephalus followed by overproduction of CSF. Studies indicated that Curcumin can inhibit ionic channels. So the aim of this study was to determine that Curcumin can reduce the AQP1 protein in Choroid plexus cells. Materials and Methods: In this study Choroidal epithelial cells of lateral ventricle were isolated from Wistar rats brain and grown in DMEM supplement. 20Μm, 30μM of Curcumin were added to cell culture medium after 24hs and also 10 μM and 15 μM of this component were added to medium after 48 hours. Effect of Curcumin on viability of these cells examined by MTT assay. Also the effect of Curcumin on the level of AQP1 protein was investigate by flow cytometry assay. Results: Based on MTT assay results, Curcumin inhibited Choroid plexus cells growth with IC50 value of 40μM and 22μM after 24 and 48 hours respectively. Flow cytomerty results indicated that Curcumin causes 50% decrease of AQP1 level in choroidal epithelial cells on dose and time-dependent manner. Conclusion: According to this study Curcumin can reduce the AQP1 protein in CP cells and this substance as a herbal medicene maybe able to be effective in treatment of some diseases such as intracranial hypertension, systemic hyponatremia and hydrocephalus by decrease CSF production.
Full-Text [PDF 361 kb]   (2162 Downloads)    
Type of Study: Research |
Received: 2012/06/9

Add your comments about this article : Your username or email:
Write the security code in the box

Send email to the article author


© 2015 All Rights Reserved | Yafteh

Designed & Developed by : Yektaweb