Showing 6 results for Jafarzadeh
Farhad Shahsavar , Mehrzad Jafarzadeh , Behnam Asadifar , Toomaj Sabooteh , Mina Darand ,
Volume 15, Issue 1 (5-2013)
Abstract
Although it is thought that drug abuse causes changes to thyroid function, the exact mechanism is still not well defined. Therefore, after a preliminary study, the aim of this supplementary study was to investigate the effect of narcotic addiction on thyroid function by measuring TBG. Materials and Methods: This research was conducted as a case - control study on 106 narcotic addicts and 106 healthy controls in Tehran. For measurement of TBG were used from serum samples of these individuals. Results: The TBG amount in case group was significantly increased compared with the control group (p<0.005). Discussion and Conclusion: The result of this supplementary study showed that the narcotic addiction can affect the thyroid function by increasing TBG. Indeed, this finding confirmed the main result of our preliminary study.
Farhad Shahsavar, Tomaj Sabooteh , Shahab Forutani, Mehrzad Jafarzadeh , Behnam Asadifar,
Volume 15, Issue 3 (9-2013)
Abstract
Introduction: Killer-cell Immunoglobulin-like Receptors (KIR) are the members of inhibitory and activating receptors expressed chiefly by the natural killer cells (NK). KIR proteins operate as receptors detecting human leukocyte antigen class Ι molecules (HLA). KIRs and their class Ι HLA ligands contribute in the pathogenesis of many kinds of diseases. The aim of this study was to genotypic analysis of KIR/HLA in the Lur population for the first time.
Materials and Methods: In this study, 100 unrelated healthy Lur individuals were KIR and HLA ligand typed by polymerase chain reaction-sequence specific primers genotyping assay. Finally, the frequency of KIR and HLA ligand genes and genotypes, as well as KIR/HLA combinations in the Lur population was compared with the Iranian population.
Results: Twenty two KIR genotypes and all of the KIR genes were observed in the Lur population. The most prevalent non-framework genes were KIR2DL1 and KIR2DP1 with the frequency of 98% and KIR3DL1 and KIR2DS4 with the frequency of 96% in the Lur population. AA genotype with the frequency of 29% was the most frequent KIR genotype ever regarded in the Lur population. The number one genotype with the frequency of 35% was the most frequent genotype in the Lur population among HLA ligand genotypes. The most common inhibitory and activating KIR/HLA combinations were KIR2DL2/3+HLA-C1 with the frequency of 75% and KIR2DS2+HLA-C1 with the frequency of 47%, respectively.
Discussion and Conclusion: The results illustrate that the frequency of KIR genes and genotypes, HLA ligands and the KIR/HLA combinations has total similar features with the Iranian population, but it is still unique by increasing or decreasing of some frequencies in the Lur population.
Farhad Shahsavar, Toomaj Sabooteh, Mehrzad Jafarzadeh ,
Volume 15, Issue 5 (3-2014)
Abstract
Background: Apolipoprotein E (ApoE) contributes to susceptibility to many different kinds of diseases. The aim of this study was genotypic analysis of ApoE in the Lur population for the first time.
Materials and Methods: In this study, 100 unrelated healthy Lur individuals were ApoE polymorphisms typed by polymerase chain reaction based restriction fragment length polymorphism (PCR-RFLP). Finally, the allele and genotype frequency of ApoE polymorphisms in the Lur population was compared with the Iranian population.
Results: Six ApoE genotypes and all of the ApoE polymorphisms were observed in the Lur population. genotype with the frequency of 48% was the most frequent genotype in the Lur population. Also, the most common allele in the Lur population was .
Conclusion: The results indicate that the frequency of ApoE polymorphisms has total similar features reported in the Iranian population, but it is still unique by increasing or decreasing some frequencies in the Lur population.
Farhad Shahsavar , Mehrzad Jafarzadeh , Maryam Soleimani Nejad , Shahab Forutani , Alireza Salekmoghadam ,
Volume 16, Issue 2 (9-2014)
Abstract
Background : Hodgkin lymphoma is a type of immune system malignancies, constituting about 30% of all cases of lymphomas. It is divided into four subgroups based on histopathologic findings. Recent reports indicate that SDF1 chemokine plays a major role in the development, survival and metastasis of many cancers, including breast, prostate and pancreas. So far, its expression and especially receptors namely CXCR4 and RDC1 has not been investigated in Hodgkin lymphoma. Therefore, in this study for the first time, SDF1 receptors expression was analyzed in the patients with Hodgkin lymphoma.
Materials and Methods: In this study, the slides were obtained from the biopsies of 40 patients with Hodgkin lymphoma. The availability of SDF1 receptors were examined using immunohistochemistry. Also, the association between these molecules with subgroup of disease was assessed by comparing the immunohistochemical findings in the expression of CXCR4 and RDC1 with the clinical data.
Results: Both SDF1 receptors are expressed on the surface of Hodgkin and Reed-Sternberg cells. CXCR4 is expressed not only on the surface of Hodgkin and Reed-Sternberg cells but also on the normal and reactive cells surrounding the tumoral cells. But RDC1 is expressed only on the surface and within the cytoplasm of tumoral cells.
Conclusion: The results of this study showed that RDC1 could be a marker for diagnosis of Hodgkin lymphoma. However, further studies using larger sample sizes and more accurate techniques are needed to confirm these results.
Mehezad Jafarzadeh, Kazem Mousavizadeh, Mohamad Taghy Joghataie, Mohsen Asghari ,
Volume 19, Issue 2 (10-2017)
Abstract
Background: Today, breast cancer is the biggest health threat to women. The current common treatments include surgery, chemotherapy, and radiotherapy. In most cancers, metastasis is the primary cause of treatment failure. Surgery and radiotherapy are effective on local tumors, but they cannot affect metastatic cancers. Chemotherapy is often used to treat metastatic cancers, the effectiveness of which is basically limited due to its toxic side effects at high doses. Due to the side effects of chemotherapy drugs, peptide therapy has become increasingly popular in the world. Accordingly, this study aims to investigate the effect of a designed bFGF antagonist peptide on inhibition of tumor growth in 4T1 metastatic breast cancer model and the serum levels of interleukin-8 and tumor necrosis factor-alpha.
Materials and Methods: Female Balb/c mice (5-7 weeks old) were used in this study. Tumors were established using 4T1 tumor transplantation method. Treatment began after two weeks, when tumors reached an appropriate size. After that, the designed peptides at three selected doses were injected intraperitoneally for 14 days. Positive and negative controls were also used for each injection. The mice in the positive and negative control groups were injected with PBS and doxorubicin, respectively. Tumors size was measured every other day using a digital caliper, and their volume was measured using the formula: length × width
2 × 0.5. After blood samples were taken from the mice’s eyes and plasma isolation was performed, interleukin-8 (IL-8) and tumor necrosis factor-alpha were measured by ELISA.
Results: The data were analyzed by one-way analysis of variance (ANOVA) using SPSS. Given the value of
p≤0.05, it can be concluded that peptide injection is effective in the reduction or inhibition of tumor growth. Significant differences are observed among the negative control group positive contro, group, and the peptide-treated group.
Conclusion: The results of this study showed that the anti-angiogenic peptide effectively inhibited the growth of the breast cancer and the results indicate that compared with the negative control group, serum levels of interleukin-8 and tumor necrosis factor-alpha are significantly different.
Abolfazl Jafarzadeh Baghan, Maghsoud Peeri, Mohammad Ali Azarbayjani,
Volume 21, Issue 3 (11-2019)
Abstract
Background: The purpose of the present study was to investigate the effect of four weeks of voluntary and compulsory exercise training in adolescence on pseudo-anxiety behaviors and its effect on the expression of fetal inflammatory factors in the cerebellum.
Materials and Methods: To conduct the experiment, 40 male rats were randomly divided into 5 groups of the control (C), separated from the mother (MS), separated from the mother and voluntary exercise (MS + RW), separated from the mother and compulsory exercise (MS + TM), and isolated from the mother and Fluoxetine (MS + FLX). Except for the control group, the rats of other groups were separated from their mothers from the second to the 14th day after birth for 180 minutes. Subsequently, the subjects were kept in cages of four rats until the 28th day. Drug and treatment groups were treated with the voluntary procedure (running wheel), compulsory practice (treadmill) and drug (Fluoxetine) from the 28th day. On the 60th day, pseudo-anxiety behaviors were evaluated by EPM behavior test. The level of the gene expression of inflammatory factors in the cerebellum was also measured.
Results: Our results showed that voluntary exercise could significantly and more effectively than compulsory training eliminate anxiety-like behaviors induced by the stress of separation from the mother from the mother, and reduce the expression of the inflammatory factors’ gene in the cerebellum.
Conclusion: The results of this study indicated that voluntary exercise can be useful as a non-prescriptive therapeutic approach against mental and psychological disorders such as anxiety.
