Showing 7 results for Shahzamani
Hamed Esmaeel Lashgarian , Kiana Shahzamani , Jamshid Raheb ,
Volume 15, Issue 1 (5-2013)
Abstract
Background : Cholesterol oxidase (CHO) is an enzyme that catalyzes cholesterol oxidation and produces hydrogen peroxide (H2O2). This enzyme is produced by certain pathogenic and non-pathogenic microorganisms and is a commercially important enzyme in the world which has found wide application in various industries. The aim of the study was to isolate native bacteria producing CHO and their identification using microbial, biochemical and molecular methods.
Materials and Methods: A total of 187 samples were collected from wastewater of leather and soap factories, soil, dairy products, and stagnant water. After the samples were cultured, the bacteria were identified using the microbial and biochemical tests. Then, the methods of colorimetric and browning of culture medium were applied to select the colonies producing CHO.PCR 16s rRNA technique was used for final confirmation of obtained strains.
Results: Of the 187 collected samples, two microbial samples were isolated, both of which from the soil samples. The results of the biochemical tests showed that the two bacteria were Rhodococcus. Both methods of colorimetric and browning of culture medium confirmed the activity of CHO. Molecular identification of these strains were also confirmed by sequencing 16s rRNA gene.
Conclusion: In this study, native bacteria producing CHO were isolated. These two bacteria were classified as Rhodococcus using morphological, biochemical and molecular methods.
Somaye Sabzali , Salar Bakhtiyari , Karemeh Haghani , Arman Rostamzad , Kyana Shahzamani ,
Volume 16, Issue 2 (9-2014)
Abstract
Background: Treatment of bacterial diseases by synthetic antibiotics leads to problems such as side effects in human and antibiotic resistance in microorganisms. Recently, plants have been given more attention in curing bacterial diseases. Since the use of Thymbra spicata is common in Iranian ancient medicine, this study tried to investigate the antibacterial effects of the extract of Thymbra speculate on some gram positive and gram negative pathogenic bacteria.
Materials and Methods: The mentioned plant was collected from Zagros mountains, Ilam province, Iran. After naming and identification, the plant extract was provided by the maceration method. The bacteria were affected by different concentrations of the extract using the disc diffusion method. Diagnostic antibiotics were used as positive control and DMSO as negative control. MIC and MBC were also determined.
Results: The highest effect of the extract was found in gram positive bacteria. The maximum zones of inhibition were observed in 76 mg/ml concentration of the extract, and S. aureus and S. epidermidis were more sensitive than other bacteria (P˂0.05). The lowest MIC was pertaining to S. aureus (1885) with 2.5 mg/ml and S. epidermidis (2405) with 5mg/ml of extract. E. coli and K. penemonia with 15 mm zone of inhibition were the most sensitive gram negative bacteria to the extract.
Conclusion: The hydroalcoholic extract of T. spicata had a considerable antibacterial effect. Statistic assessment of the inhibition zones showed that the extract was more effective on gram positive bacteria
Shahrbano Oryan , Mohammad Nabiuni , Masoud Bagherpour Zarchy , Morteza Nazari Serenje , Ali Bagherpour Zarchy , Kina Shahzamani ,
Volume 17, Issue 2 (9-2015)
Abstract
Background : The Alzheimer is a common progressive and degenerative disease which causes dementia and ruin of brain cells especially in Hippocampus region. Recently, it has been appeared that Erythropoietin with its neural-protective action causes an improvement in cognitive functions during this disease. This study aims to find an alternative approach instead of traditional intraventricular injection to deliver Erythropoietin into the brain without passing the blood-brain barrier. Materials and Methods: In order to induce Alzheimer in rats, the STZ was injected in lateral ventricles of brain. Two weeks after Alzheimer induction, different doses of erythropoietin were injected in sisternamagna for treatment of the disease with decreased side effects. After the treatment period, all rats were tested by passive avoidence learning test using shuttle box, and measuring of blood hematocrit. Results: Although the Erythropoietin did not have significant influence on improvement of memory and learning phenomena in both control and sham groups, it resulted in a significant difference in improvement of the disease comparing STZ group that this treatment was accompanied with decreased hematocrit than the group receiving 5000 U/Kg doses. Conclusion: These results suggested that injection of STZ in lateral ventricles of brain contributes to extreme damage in memory and learning similar to Alzheimer on the other hand, Erythropoietin prevents such a damage accompanied with decreased hematocrit.
Kiyana Shahzamani, Hamed Esmaeil Lashgarian , Omid Ali Papi, Hamid Mokhayeri,
Volume 18, Issue 1 (5-2016)
Abstract
Background : Molecular diagnostic methods are among major tools in management of hepatitis C virus (HCV) in infected patients. Many studies have shown that viral load is associated with stage of infection and response to treatment. Therefore, the evaluation and quantification of viral load is very important. The goal of this study is implementation of inexpensive, yet accurate method for quantitative assessment of viral load in plasma samples of infected patients.
Materials and Methods: After development and validation of the assay, quantification of HCV RNA on 200 chronic patients the start of therapy was performed using an In-House Real-time PCR assay. Measuring the concentration of viral RNA was performed using an external standard curve. It should be noted that the validation and standardization of all procedures in this study were performed using RNA standard panel. The results of this method were compared with results obtained from Artus commercial kit.
Results: Detection limit of the assay was 50 IU/ml. The mean viral load measured on a logarithmic scale (5/81± 0/22, p<0/05). Parallel analysis of samples with commercial kit showed that there is a good correlation between these two methods (R2 = 0.988 p< 0.05).
Conclusion: Viral load detection of HCV was reported for the first time in Khorramabad city. According to the results, this method has a good sensitivity and specificity for HCV quantification in large-scale. It can be a good replacement for commercial kits especially for clinical evaluation of therapy.
Sayad Khanizadeh, Kiyana Shahzamani, Hadi Razavi Niko, Hmed Esmaiili,
Volume 18, Issue 2 (9-2016)
Abstract
Background : Hepatitis C virus (HCV) is considered as one of the major pathogenic agents of chronic liver diseases. Previous studies have shown that HCV proteins can interaction with gene regulatory networks such as microRNAs. The aim of this study was to investigate the effect of HCV core protein on the expression of miR-150 in a cell culture model.
Materials and Methods: Plasmids expressing full HCV core protein was transfected into Huh7 cell lines while a GFP expressing plasmid employed as negative control. Subsequently, total RNA extracted and Real-Time PCR performed to measure the expression level of miR-150 expression. Moreover, trypan blue exclusion assay was performed to investigate the effect of core protein on cell viability.
Results: The gene expression analysis of miR-150 in Huh7 cells showed that endogenous HCV core protein could significantly down regulation of miR-150 when compared to GFP control plasmid and normal cells (P<0.01). Beside, core protein induced no significant proliferative or cytotoxic effects on hepatic cells as determined by trypan blue exclusion assay (P<0.05).
Conclusion: Our study suggests that HCV core protein can led to down regulation of miR-150 expression. This data revealed that HCV protein interactions with cell regulatory machinery may contribute to pathogenesis of chronic liver diseases.
Somaieh Sabzali, Kiana Shahzamani, Hamzeh Hasani, Mehdi Birjandi,
Volume 26, Issue 2 (6-2024)
Abstract
Background: The presence of microbial agents on different hospital surfaces is considered a harmful factor to health. The use of hypochlorous acid (HOCl) for health purposes has been evaluated in more than 50 countries. The present study aimed to assess the disinfection effect of Sanitizon solutions and common disinfectants (Sayasept HP and Sayasept 420) on the reduction of microbial load in Shohada-ye Ashayer Hospital, Khorram Abad.
Materials and Methods: In this experimental study, 200 samples from intensive care units were prepared and studied in seven experimental groups (one control group and six different types of disincentives) in six different hospital departments. The samples were cultured on specific culture media. The results were analyzed using SPSS software (version 23). The germicidal power of disinfectants was investigated according to standard 10504 and diffusion method in agar.
Results: The use of disinfectants caused a significant decrease in the microbial load compared to the control group. Disinfection solutions led to a decrease in the number of colonies from 113 to 12. Moreover, all disinfectants had germicidal and fungicidal power according to the standard (reduction of four logarithms of bacteria). The Sanitizon solution succeeded in removing all microorganisms and reducing more than seven logarithms of test microorganisms. Sanitizon caused a more marked reduction of gram-negative bacteria and fungi compared to other disinfectants.
Conclusion: As evidenced by the results, Sanitizon can be used along with other common disinfectant products to clean surfaces. The intermittent use of disinfectants with different active substances reduces the probability of resistance in microorganisms.
Somaieh Sabzali, Seifollah Bahramikia, Kiana Shahzamani,
Volume 26, Issue 4 (10-2024)
Abstract
Background: An increase in blood fat, especially cholesterol, is one of the chronic diseases that are caused by metabolic disorders caused by many factors. Due to the side effects and harmful effects of chemical drugs, the issue of returning to the use of herbal and natural drugs has received serious attention nowadays. Therefore, the present study aimed to introduce and review different formulations of herbal extracts whose effectiveness in the reduction of blood lipid profile has been proven.
Materials and Methods: The studies conducted in this field were reviewed in the form of a narrative review article.
Results: It is noteworthy that the desire to use medicinal plants that reduce blood fat has become widespread in most societies, even in developed countries. The consumption of these plants is especially significant when common treatments are not able to control the disease.
Conclusion: Although the arbitrary consumption of medicinal plants by patients with high blood fat may improve the disease, there is a possibility of drug interaction with common drugs, and because its use is irregular and with an uncertain and variable dose, it may cause a disturbance in disease control. Therefore, if it is intended for use, the treating physician must be informed.
