Behrouz Yarahmadi , Hamid Reza Mahdavi , Azam Moaydinezhad ,
Volume 10, Issue 3 (yafte 2008)
Abstract
Yarahmadi B1, Mahdavi HR2, Moaydinezhad A4 1. Research Instructor, Department of Animal Products, Lorstan Research Center of Agriculture and Natural Resources, Khorramabad, Iran 2. Research Assistant Professor, Department of Animal Products, Karaj Animal Science Research Institue, Karaj, Iran 3. MSc in Biotechnology, Department of Agricultural Biotechnology, Hamadan Bou Ali University, Hamadan, Iran Abstract Background: The primary quality of raw milk is affected by several factors including animal nutrition, animal health, milk components, and its bacterial activity and these factors are effective on the final products. Therefore, the quality of raw milk depends on time and milk condition from production to delivery. In Lorestan, 97% of the milk production is obtained from rural and small units. This study was carried out to determine the factors affecting the bacteriological quality of raw milk from letdown phase till delivery to factory and to find out the critical points of bacterial contamination in the years 2003 and 2004. Materials and methods: In this study, the collecting centers of raw milk with a capacity of more than 3 tons, between 1.5 to 3 tons, and fewer than 1.5 tons were included as the centers with high, moderate, and low capacity respectively. The sampling stages consisted of cow milk letdown, milk collecting equipment, milk collecting tankers, milk cooling tanks, and milk delivery to factory. Microbial experiments consisted of standard plate count (SPC), E. coli, and coliforms. All the collected samples were 45 ones in each month and 540 samples in a year. Being converted to Log10, the data were analyzed by the SPSS software (version 11.5) and GLM procedure was used to determine the effects of month, capacity of centers, and sampling stages. Results: Based on the results of the study, the average total count (TC) in all seasons in Lorestan province was 6.43 0.37cfu/m. The effects of month, capacity of centers, and the sampling stages on TC, E. coli, and coliforms were statistically significant (p<0.01). Additionally, the lowest TC (6.31 0.38cfu/m) was in January while the highest (6.57 0.31cfu/m) was in August. Moreover, the decreasing trend of TC started from September and was the lowest in January and February. Correlation coefficients showed that there was a significant positive correlation between TC and E. coli (r=0.79) and TC and coliforms (r=0.90, p<0.01). Conclusion: The major problems of milk collecting in Lorestan province are the long distances of some milk collecting centers from the milk factories as well as the long milk delivery time which play important roles in increasing TC from letdown stage till delivery to factory in addition to the insufficient road facilities in some of the villages in Lorestan province.
Hasan Ahmadvand , Farshid Ghazanfari , Reza Yarahmadi , Mona Mankavi , Rezvan Vajdian,
Volume 15, Issue 2 (8-2013)
Abstract
Background : Oxidation of lipids such as low-density lipoprotein (LDL) has been strongly suggested as a key factor in the pathogenesis of atherosclerosis. Thus the inclusion of some anti-oxidant compounds such as vitamin E and Satureja Khozistanica Essential oil (SKE) in daily dietary food stuff may inhibit the production of oxidized LDL and may decrease both the development and the progression of atherosclerosis. The aim of this research is to study inhibitory effects of different concentrations of essential oil Satureja Khozistanica on LDL oxidation in vitro.
Materials and Methods:Fasting blood samples from normal people after an overnight fasting were collected and then isolated LDL was incubated without CuSO4 as control and incubated with CuSO4 and several concentration of SKE (50, 100 and 200 mg/ml) and vitamin E as positive control (100 µM). Then the formation of conjugated dienes, lag time and thiobarbituric acid reactive substances (TBARS)were measured. Inhibition of this Cu-induced oxidation was studied in the presence of several concentration of SKE and vitamin E.
Results: The results showed that SKE induced LDL oxidation and decrease the resistance of LDL against oxidation in vitro. SKE at concentrations of 50, 100 and 2000 µg/ml, and vitamin E (100 µM) showed an increase rate of 33.33, 66.66, 100.00 and 111.11% respectively, against oxidation in vitro. The inhibitory effects of the SKE on LDL oxidation were dose-dependent at concentrations of 50, 100 and 200 µg/ml.
Conclusion: This study showed that Satureja Khozistanica prevented the oxidation of LDL in vitro and it may suggest that it has the similar effects in vivo.
Mohammad Nabiuni , Azam Yarahmadi , Bahram Delfan ,
Volume 15, Issue 4 (1-2014)
Abstract
Background: Acute promyelocytic leukemia is the most malignant type of myeloid leukemia characterized by chromosomal translocation (15 and 17) and also blocking the cells in promyelocytic stage of differentiation into myeloid. Nowadays, differentiation therapy is used to treat leukemia. Previous studies indicate that vitamin E inhibits proliferation and also induces differentiation of HL-60 cell line towards monocyte. Since high concentrations of vitamin E to induce differentiation have many side effects, the search for alternative compounds is inevitable. Regarding anti- proliferative and anti-cancer effect of bee venom (BV), in this study the effect of BV on alpha tocopheryl succinate function in differentiation was examined. Materials and Methods: In this study cellular differentiation was tested by immunocytochemistry ,Wright-Giemsa staining and NBT reduction.Data were analyzed using one-way ANOVA test and Instate 3 software. Results: The results showed that BV in non-toxic concentrations can increase the differentiation potency of vitamin E on HL-60 cancer cell line. Conclusion: Non- toxic concentration of BV can increase differentiational effects of vitamin E and it is expected that BV can increases the differentiating potential of differentiator components in the future .
