Showing 5 results for Curcumin
Mohammad Nabiuni , Bahram Delfan, Zahra Nazari, Abdolhamid Angaji ,
Volume 14, Issue 1 (6-2012)
Abstract
Background : Aquaporin1 (AQP1) protoin channels that expressed in the Choroid plexuses of brain ventricles, have an important role in cerebrospinal fluid (CSF) production. Some pathophysiological conditions such as intracranial hypertension, systemic hyponatremia and hydrocephalus followed by overproduction of CSF. Studies indicated that Curcumin can inhibit ionic channels. So the aim of this study was to determine that Curcumin can reduce the AQP1 protein in Choroid plexus cells.
Materials and Methods: In this study Choroidal epithelial cells of lateral ventricle were isolated from Wistar rats brain and grown in DMEM supplement. 20Μm, 30μM of Curcumin were added to cell culture medium after 24hs and also 10 μM and 15 μM of this component were added to medium after 48 hours. Effect of Curcumin on viability of these cells examined by MTT assay. Also the effect of Curcumin on the level of AQP1 protein was investigate by flow cytometry assay.
Results: Based on MTT assay results, Curcumin inhibited Choroid plexus cells growth with IC50 value of 40μM and 22μM after 24 and 48 hours respectively. Flow cytomerty results indicated that Curcumin causes 50% decrease of AQP1 level in choroidal epithelial cells on dose and time-dependent manner.
Conclusion: According to this study Curcumin can reduce the AQP1 protein in CP cells and this substance as a herbal medicene maybe able to be effective in treatment of some diseases such as intracranial hypertension, systemic hyponatremia and hydrocephalus by decrease CSF production.
Mohamad Nabiuni, Homa Kouchesfahanii, Sakineh Azari, Bahram Delfan,
Volume 19, Issue 2 (10-2017)
Abstract
Background:Digestive system is the most common type of cancer in Iran with 38 percent of the cases.The risk of this cancer is influenced by both environmental and genetic factors. Curcumin is kind of phytochemicals which is important in chemoprevention strategy, in order to slow, block, or reverse the process of carcinogenesis.Researchhave shown that the possible role of curcumin to prevent or delay the diagnosis of colorectal cancer. In this research the effect of curcumin on proliferation of HT_29 cancerous cells were assayed.
Materials and Methods:In this experimental research HT_29 cell line were cultured in DMEM medium containing 10% FBS (Fetal Bovine Serum, Gibco, Invitrogen) and 100 U/ml penicillin and 100mg/ml streptomycin. Different concentrations of curcumin on the growth of HT_29 cells were determined by MTT assay and the type of death induced was assessed by DAPI staining method.All experiments were done three times and data were analyzed byone –way ANOVA test and Instate 3 software.
Results:The cell growth-inhibiting rate was about 53% at 50μM curcumin concentration after 24h of treatment(IC50). Vacuolation and significant decrease of cells were observed after 72h of treatment. Moreover, induction of apoptosis was observed with the DAPI staining method.
Conclusion:According to molecular mechanisms of cell proliferation and curcumin ability in the induction of pro_apoptotic proteins and the inhibition of anti_apoptotic proteins as well as inhibition of as survival pathways,like NF_KB and AKT, this predisposition makes curcumin a good anticancer drug.
Hoseyn Dalvand, Ahmad Hematfar, Naser Behpoor,
Volume 21, Issue 1 (5-2019)
Abstract
Background: Curcumin is an active ingredient in turmeric, which is used as herbal medicine for the treatment of certain diseases. The aim of this study was to investigate the effects of two weeks of exhaustive swimming and supplementation of curcumin on alcohol induced liver damage biomarkers in male wistar rats.
Materials and Methods: In this study, 32 male wistar rats were randomly selected and divided into 4 equal groups: control, training, supplemental curcumin, and training with curcumin supplement. At first, every 8 hours for 4 days, alcohol was gavaged to all groups, proportional to the body weight of each rat. This was followed by an alcohol withdrawal period. After that, the practice period began, including long-term swimming in water, for the exercise group, and the curcumin supplement with exercise group. Finally, blood samples were taken from the heart under anaesthetic
Results: Curcumin had no significant effect on AST (P = 0.401) and ALT (P = 0.978) and the ratio of these two enzymes (p = 0.657). Exercise significantly reduced AST (P = 0.022), but did not significantly decrease ALT (P = 0.759) or the ratio of these two enzymes (p = 0.225). Exercise and supplementation interaction did not significantly decrease ALT (P = 0.462) or AST (P = 0.073) activity or the AST / ALT ratio (P = 0.520).
Conclusion: The reduction of liver damage markers in this study suggests that exercise and curcumin consumption may, through protective effects, improve the negative effects of alcohol on the liver, and prevent alcohol induced liver disease.
Mohammad Mahdi Madani, Bahram Abedi,
Volume 23, Issue 0 (11-2021)
Abstract
Background: Obesity is associated with a chronic inflammation that plays a major role in the development of metabolic disorders or insulin resistance. The aim of this study was to evaluate the effect of 8 weeks of aerobic exercise with curcumin supplementation on proinflammatory factors in obese men.
Materials and Methods: 20 obese men in the age range of 30 to 40 years were purposefully selected and randomly divided into two equals experimental (supplementary) and control (placebo) groups. The experimental group participated in a moderate-intensity aerobic exercise program (60-70% of maximum heart rate) for three weeks, three sessions per week, and each session lasted about 60 minutes. At the same time, they took 500 mg of curcumin daily in capsule and placebo groups. The control group also took a placebo (rice flour). Before and after the supplementation training intervention, blood samples were taken from all subjects during 10 hours of fasting. Inflammatory markers including TNFα, IL-1β and BDNF were assessed by ELISA. Data were analyzed using analysis of covariance (p <0/05).
Results: The results showed that eight weeks of aerobic exercise with curcumin supplementation, in addition to improving body composition, caused a significant decrease in TNFα (p = 0/0001) and IL-1β (p = 0/0001) and also a significant increase in BDNF values (p = 0/0001) in obese men.
Conclusion: The findings of this study show that curcumin supplementation along with moderate-intensity aerobic exercise can improve inflammatory conditions in obese men.
Keywords: Obesity, Inflammation, Curcumin, Aerobic exercise.
Amin Ghanbariani Gandom Bani, Mohamad Fathi, Rahim Mirnasouri, Elham Goodarzi,
Volume 26, Issue 2 (6-2024)
Abstract
Background: Physical activity with anti-inflammatory and antioxidant effects can play a critical role in the health of individuals. Therefore, the present study aimed to assess the impact of resistance training and curcumin consumption on inflammatory indicators and oxidative stress in obese men.
Materials and Methods: The present study was conducted based on a quasi-experimental design. The studied population consists of obese men aged 38-43 years. The sample size was estimated to be 40 individuals, who were randomly assigned to four groups of 10 using a random number table. The training program lasted for eight weeks, with three sessions per week. An 80 mg curcumin supplement was administered. Blood samples were collected before and after the final session. Data analysis involved one-way analysis of variance, paired t-test, and Tukey's post hoc test at a significance level of 0.05.
Results: The amount of catalase enzyme in resistance training groups (P=0.012), curcumin supplement (P=0.032), and resistance training group with curcumin consumption (P<0.001) were associated with a significant increase. Nonetheless, it was not significant in the control group (P=0.991). Furthermore, the levels of malondialdehyde, interleukin-6, C-reactive protein, and tumor necrosis factor-alpha in the experimental groups displayed a significant decrease (P<0.05), whereas these changes were not significant in the control group (P<0.05).
Conclusion: As evidenced by the obtained results, it can be stated that the consumption of curcumin may be effective in suppressing the inflammation caused by obesity. This is due to its potent antioxidant effects and its combination with resistance training.
