Volume 6, Issue 3 (11-2004)                   yafte 2004, 6(3): 19-28 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

rahimi A A, Shahoseini M H, Ahangari G, Shahsavar F, SeyedRezaye Tehrani S M. Fetal sex determination via Nested-PCR method using Amylogenin gene. yafte. 2004; 6 (3) :19-28
URL: http://yafte.lums.ac.ir/article-1-928-en.html
Abstract:   (11904 Views)
Background: Detection of fetal sex is valuable for parents. The aim of this study was detection of fetal sex in suspect cases recessive related to sex disease in first trimester of pregnancy. Material and method: After collecting C.V samples and separating from mother’s samples, their DNAs have been extracted, then doing PCR, finally the sex of them detected. Also, after increase in sensitivity of system will be able to detection sex of a cell that obtain by PCR. A statistical analysis was made using the fisher test. Results: sex of 74 samples chorionic villous detected by PCR only success to related with many family for following, but also the sex was correct. After increasing in sensitivity of system, detected the sixteen fetuses that was in different stage cellular. Also detected two unfertilized ovum and fertilized ovum but no divided. Conclusion: Sex detection of fetus before delivery, in first trimester of pregnancy will be preventing to new born patient babies, and also decrease the love and relation between mother and her newborn. It can be used in detection of recessive sex related disease in IVF cases for sex detecting and transfer female fetus to her mother. Another application is for control of population. This optimized molecular which designed based on amelogenin target, detect sex in blood samples C.V single cell PCR with high sensitivity & specificity.
Full-Text [DOC 151 kb]   (1856 Downloads)    
Type of Study: Research |
Received: 2012/12/25 | Accepted: 2017/11/14

Add your comments about this article : Your username or Email:
Write the security code in the box

© 2015 All Rights Reserved | Yafteh

Designed & Developed by : Yektaweb