Volume 20, Issue 4 (1-2019)                   yafte 2019, 20(4): 1-7 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sadeh M, Falsafi S, Amini K. The Prevalence of Papillomavirus-16 and -18 isolated from women with cervical cancer using Multiplex PCR. yafte 2019; 20 (4) :1-7
URL: http://yafte.lums.ac.ir/article-1-2372-en.html
Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran.
Abstract:   (6424 Views)

Background: Women infected with human papillomavirus, especially types 16 and 18, are at risk of cervical cancer. The purpose of this study was to determine the frequency of papillomavirus-16 and -18 in women with cervical cancer using multiplex-PCR.
Materials and Methods: In this experimental study, after collecting blood samples, viral DNA was extracted using a Cinaclone kit, and PCR, with specific primers, was performed to detect HPV-16 and HPV-18. PCR products were analyzed by 1% agarose gel electrophoresis.
Results: Of 60 samples, 19 were infected with HPV. The results showed that the frequency of genotype HPV-16 and HPV-18 was 8 (42.1%) and 11 (57.9%), respectively.
Conclusion: The study showed that using PCR with specific primers for the detection of HPV-16 and HPV-18 is a convenient and accurate method. The results of this study indicate the relationship between HPV and cervical cancer.

Full-Text [PDF 244 kb]   (1376 Downloads)    
Type of Study: Research | Subject: میکروب شناسی
Received: 2017/01/6 | Accepted: 2017/05/9 | Published: 2019/02/24

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Yafteh

Designed & Developed by : Yektaweb