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Department of Food Science and Technology, Sarvestan Branch, Islamic Azad University, Sarvestan, Iran.
Abstract:   (66 Views)
Background: Lipases are one of the most important catalysts for several industries such as detergent, dairy, and textile industry due to their bio-catalytic ability in aqueous and non-aqueous media. Screening different habitats for bacteria with lipase activity and optimizing its fermentation parameters will facilitate its effective production and use in industries.
Materials and Methods: In this study, wastewater samples from Golbahar Meat Products Factory of Khorramabad were screened for lipase-producing bacterial strains. The lipase-producing isolates were identified based on morphological, biochemical characterization and 16S rRNA gene sequence. The culture medium parameters such as pH, incubation time and temperature were varied for the purpose of optimization.
Results: Three isolates with high lipase activity identified by screening of wastewater samples from Golbahar Meat Products Factory of Khorramabad. The BLAST program showed the highest similarity of identified isolates 16S rRNA genes with Bacillus amyloliquefaciens.  Maximum lipase activity was recorded at 48 h incubation. Moreover, the purified lipase showed the maximum activity at 45 °C and pH = 8.0.
Conclusion: The results of this study showed that the extracellular lipases of Bacillus amyloliquefaciens isolates are alkaline lipases and have relatively good temperature stability. These extracellular lipases can be used in various industries. Furthermore, gene encoding lipase enzyme of these isolates can be useful in the enzyme engineering process and the lipase activity can be changed as desired by site-directed mutagenesis.
 
     
Type of Study: Research | Subject: میکروب شناسی
Received: 2022/05/29 | Accepted: 2022/06/28

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