Volume 25, Issue 3 (11-2023)
yafte 2023, 25(3): 23-35 |
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Rastegarvand N, Soleimanjahi H, Arefian E, Pourkarim M, Saadatpour F, Ghanbarnasab Behbahani R. Challenges of Gene Transfer to Colorectal Cancer Cells (CRC). yafte 2023; 25 (3) :23-35
URL: http://yafte.lums.ac.ir/article-1-3644-en.html
URL: http://yafte.lums.ac.ir/article-1-3644-en.html
Nasrin Rastegarvand 
, Hoorieh Soleimanjahi * , Ehsan Arefian , Mahmoudreza Pourkarim , Fatemeh Saadatpour , Rahil Ghanbarnasab Behbahani
, Hoorieh Soleimanjahi * , Ehsan Arefian , Mahmoudreza Pourkarim , Fatemeh Saadatpour , Rahil Ghanbarnasab Behbahani
Medical Virology Department, Tarbiat Modares University, Tehran, Iran
Abstract: (504 Views)
Background: Gene transfection is a powerful tool for changing cell function, which is used for treating various diseases. Nevertheless, various studies show that despite the variety of gene transfer methods, there are significant limitations in the application of each method. The present study aimed to compare two methods of gene transfer using PEI and Lipofectamine compounds in cancer cell.
Materials and Methods: In this study, Caco-2 and HCT-116 were used as target cells, and HEK-293 cells were used as control cells. For this purpose, initially, gene transfer was performed using PEI and Lipofectamine, and then their results were compared with each other based on the expression of GFP protein.
Results: The results of this study showed that although gene transfer was carried out in HEK-293 cells at a favorable rate, this process was very insignificant in colorectal cells. In addition, in the meantime, some differences were observed between the amounts of gene transfer in colorectal cells, so that the amount of transfection in Caco-2 cells was lower than that of the HCT-116 cells.
Conclusion: The degree of acceptance of foreign genes in cells depends on several factors, including the type and origin of the cell. Since cancer cells have undergone many genetic changes, their genetic manipulation is associated with many limitations. For this reason, it is recommended to use other methods, such as cell selection by antibiotics and preparation of stable cell lines to reach a high level of gene transfer in these cells.
Materials and Methods: In this study, Caco-2 and HCT-116 were used as target cells, and HEK-293 cells were used as control cells. For this purpose, initially, gene transfer was performed using PEI and Lipofectamine, and then their results were compared with each other based on the expression of GFP protein.
Results: The results of this study showed that although gene transfer was carried out in HEK-293 cells at a favorable rate, this process was very insignificant in colorectal cells. In addition, in the meantime, some differences were observed between the amounts of gene transfer in colorectal cells, so that the amount of transfection in Caco-2 cells was lower than that of the HCT-116 cells.
Conclusion: The degree of acceptance of foreign genes in cells depends on several factors, including the type and origin of the cell. Since cancer cells have undergone many genetic changes, their genetic manipulation is associated with many limitations. For this reason, it is recommended to use other methods, such as cell selection by antibiotics and preparation of stable cell lines to reach a high level of gene transfer in these cells.
Type of Study: Research |
Subject:
علوم آزمایشگاهی
Received: 2023/05/21 | Accepted: 2023/10/12 | Published: 2023/11/1
Received: 2023/05/21 | Accepted: 2023/10/12 | Published: 2023/11/1
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