Volume 26, Issue 4 (10-2024)
yafte 2024, 26(4): 13-25 |
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Hanifehpour H, Fallahi S, Ashrafi F, Siasi E. Application of One-step-RT-LAMP molecular technique in SARS-CoV-2 virus detection in nasopharyngeal samples collected from medical and reference centers of Tehran province. yafte 2024; 26 (4) :13-25
URL: http://yafte.lums.ac.ir/article-1-3690-en.html
URL: http://yafte.lums.ac.ir/article-1-3690-en.html
Hepatitis Research Center, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran & Hepatitis Research Center, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
Abstract: (151 Views)
Background: Rapid, accurate, and specific molecular diagnosis of SARS-CoV-2 virus is essential to prevent and fight against corona disease, therefore, the purpose of this research is to investigate the diagnostic value of LAMP (Loop-Mediated Isothermal Amplification) molecular technique in rapid evaluation and obtaining accurate results and reducing the costs of corona testing compared to other routine molecular methods.
Materials and Methods: The results showed, 342 nasopharyngeal samples were collected from reference medical centers in Tehran. In the next step, RNA was extracted from the samples. After designing the primer for the N gene, an RT-LAMP assay was performed. To confirm the results, LAMP products were electrophoresed on agarose gel. Also, the RT-LAMP technique was evaluated in terms of sensitivity and specificity. SPSS version 22 software was used for statistical analysis of the results.
Results: In the present study, the statistical analysis of the data showed that there is a significant difference between the gender variable and the severity of the disease (P>0.001, while there was no significant difference in terms of the age variable (P>0.05). In terms of clinical symptoms, the relationship There was a substantial difference between the severity of the virus infection with the symptoms of fever, cough and headache (P>0.001. Also, statistically, a significant difference was observed between diabetes and the severity of the disease (P>0.001) in terms of the sensitivity and specificity of the RT-LAMP technique., the sensitivity of this dilution technique was 101x1 and the specificity was 100%.
Conclusion: Fast and timely diagnosis of the Corona virus is very important due to the high rate of spread of the virus. Therefore, there is a need for a fast and cheap diagnostic method that can be performed in different places and is affordable with high sensitivity and specificity. Based on the results obtained from the present study, the RT-LAMP technique is a fast, sensitive, and effective method for diagnosing SARS-CoV-2.
Materials and Methods: The results showed, 342 nasopharyngeal samples were collected from reference medical centers in Tehran. In the next step, RNA was extracted from the samples. After designing the primer for the N gene, an RT-LAMP assay was performed. To confirm the results, LAMP products were electrophoresed on agarose gel. Also, the RT-LAMP technique was evaluated in terms of sensitivity and specificity. SPSS version 22 software was used for statistical analysis of the results.
Results: In the present study, the statistical analysis of the data showed that there is a significant difference between the gender variable and the severity of the disease (P>0.001, while there was no significant difference in terms of the age variable (P>0.05). In terms of clinical symptoms, the relationship There was a substantial difference between the severity of the virus infection with the symptoms of fever, cough and headache (P>0.001. Also, statistically, a significant difference was observed between diabetes and the severity of the disease (P>0.001) in terms of the sensitivity and specificity of the RT-LAMP technique., the sensitivity of this dilution technique was 101x1 and the specificity was 100%.
Conclusion: Fast and timely diagnosis of the Corona virus is very important due to the high rate of spread of the virus. Therefore, there is a need for a fast and cheap diagnostic method that can be performed in different places and is affordable with high sensitivity and specificity. Based on the results obtained from the present study, the RT-LAMP technique is a fast, sensitive, and effective method for diagnosing SARS-CoV-2.
Type of Study: Research |
Received: 2023/11/14 | Accepted: 2024/10/22 | Published: 2024/10/31
Received: 2023/11/14 | Accepted: 2024/10/22 | Published: 2024/10/31
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