Volume 26, Issue 2 (6-2024)
yafte 2024, 26(2): 71-85 |
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Rahmani Moghadam E, Karbalay-doust S, Ghalavandi M, Erfanizadeh M, Namavar M R. Effect of chronic sleep deprivation on the structural changes of the anterior nucleus of the thalamus and mammillary bodies in male rats: A stereological study. yafte 2024; 26 (2) :71-85
URL: http://yafte.lums.ac.ir/article-1-3721-en.html
URL: http://yafte.lums.ac.ir/article-1-3721-en.html
Ebrahim Rahmani Moghadam 
, Saied Karbalay-doust , Mehrnaz Ghalavandi , Mahboobeh Erfanizadeh , Mohammad Reza Namavar *
, Saied Karbalay-doust , Mehrnaz Ghalavandi , Mahboobeh Erfanizadeh , Mohammad Reza Namavar *
Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran & Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract: (535 Views)
Background: Sleep deprivation, a common problem in modern life, can induce oxidative stress and inflammatory responses, exerting adverse effects on cognitive functions. These injuries may be the result of cellular and molecular changes in some areas of the brain, such as the frontal nucleus of the thalamus and mammary bodies, which have received assiduous attention. These nuclei participate in learning, memory, and emotional (and recently sleep) functions. The present study aimed to assess the effect of chronic sleep deprivation on structural changes in these areas.
Materials and Methods: A total of 21 adult male rats were randomly assigned to three groups: control, control-grid, and sleep deprivation, and examined for 21 days. Brains were removed and fixed with buffered formalin. Brains were serially and systematically cut and stained with Giemsa. The total volume of nuclei and their total number of neurons and non-neurons were unbiasedly estimated by stereological methods.
Results: The findings of this study demonstrated that chronic sleep deprivation reduced the total volume of all nuclei (anterior nuclei of the thalamus and mammillary bodies of the hypothalamus) when compared with control groups (P<0. 05). Moreover, the total number of neurons and non-neurons in these structures significantly decreased in sleep-deprived animals when compared with control groups.
Conclusion: Chronic sleep deprivation has detrimental effects on the central nervous system and limbic system, as well as on the anterior nucleus of the thalamus, which, until recently, was believed to be not involved in sleep. These effects could be partly due to a decrease in cell (neuron and non-neuron) number, resulting in functional loss of this system.
Materials and Methods: A total of 21 adult male rats were randomly assigned to three groups: control, control-grid, and sleep deprivation, and examined for 21 days. Brains were removed and fixed with buffered formalin. Brains were serially and systematically cut and stained with Giemsa. The total volume of nuclei and their total number of neurons and non-neurons were unbiasedly estimated by stereological methods.
Results: The findings of this study demonstrated that chronic sleep deprivation reduced the total volume of all nuclei (anterior nuclei of the thalamus and mammillary bodies of the hypothalamus) when compared with control groups (P<0. 05). Moreover, the total number of neurons and non-neurons in these structures significantly decreased in sleep-deprived animals when compared with control groups.
Conclusion: Chronic sleep deprivation has detrimental effects on the central nervous system and limbic system, as well as on the anterior nucleus of the thalamus, which, until recently, was believed to be not involved in sleep. These effects could be partly due to a decrease in cell (neuron and non-neuron) number, resulting in functional loss of this system.
Keywords: Anterior nucleus of the thalamus, Limbic system, Mammillary bodies, Sleep deprivation, Stereology
Type of Study: Research |
Subject:
داخلی
Received: 2024/04/12 | Accepted: 2024/07/13 | Published: 2024/06/30
Received: 2024/04/12 | Accepted: 2024/07/13 | Published: 2024/06/30
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