Volume 26, Issue 4 (10-2024)
yafte 2024, 26(4): 26-36 |
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Keyhani A, Sharifi I, Ezatpour B, Sakifar F, Mahmoudvand H. Effect of Nectaroscordeum koelzii ethanolic extract on the growth and induction of apoptosis in Leishmania tropica promastigotes. yafte 2024; 26 (4) :26-36
URL: http://yafte.lums.ac.ir/article-1-3770-en.html
URL: http://yafte.lums.ac.ir/article-1-3770-en.html
Department of Parasitology and Mycology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran & Department of Parasitology and Mycology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
Abstract: (181 Views)
Background: This study aims to investigate the in vitro effects of ethanolic extract of Nectaroscordeum koelzii on the growth and induction of apoptosis in promastigotes of Leishmania tropica.
Materials and Methods: A growth inhibition test was conducted to evaluate the effects of the extracts and a control drug on human macrophage cell lines (THP-1) and promastigotes of the standard strain of Leishmania tropica (MHOM/IR/2002/Mash2), which was obtained from the Leishmaniasis Research Center of Kerman University of Medical Sciences. The MTT method (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was used for this evaluation. Additionally, the activity of the caspase-3-like enzyme in promastigotes was assessed using colorimetric Caspase-3 activity assay kits (Sigma-Aldrich, Germany), following the manufacturer's instructions.
Results: The ethanolic extract of N. koelzii significantly reduced the viability of L. tropica promastigotes (P < 0.001), with an inhibitory concentration 50 (IC50) value of 88.7 μg/ml. The findings also indicated that the extract significantly increased the activation of the caspase-3 enzyme (P < 0.01). the cytotoxic concentration 50 (CC50) values for the ethanolic extract of N. koelzii and glucantime on THP-1 macrophage cells were 496.9 μg/ml and 1215.2 μg/ml, respectively.
Conclusion: The results of the present study indicate the potential anti-leishmanial effects of the ethanolic extract of N. koelzii on the promastigotes of Leishmania tropica. Induction of apoptosis may also be one of the most significant cellular mechanisms underlying the anti-parasitic effects of this extract. Additionally, the findings demonstrate that this extract exhibits low cytotoxicity towards normal human cells. However, further studies are needed to investigate the exact cellular mechanisms.
Materials and Methods: A growth inhibition test was conducted to evaluate the effects of the extracts and a control drug on human macrophage cell lines (THP-1) and promastigotes of the standard strain of Leishmania tropica (MHOM/IR/2002/Mash2), which was obtained from the Leishmaniasis Research Center of Kerman University of Medical Sciences. The MTT method (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was used for this evaluation. Additionally, the activity of the caspase-3-like enzyme in promastigotes was assessed using colorimetric Caspase-3 activity assay kits (Sigma-Aldrich, Germany), following the manufacturer's instructions.
Results: The ethanolic extract of N. koelzii significantly reduced the viability of L. tropica promastigotes (P < 0.001), with an inhibitory concentration 50 (IC50) value of 88.7 μg/ml. The findings also indicated that the extract significantly increased the activation of the caspase-3 enzyme (P < 0.01). the cytotoxic concentration 50 (CC50) values for the ethanolic extract of N. koelzii and glucantime on THP-1 macrophage cells were 496.9 μg/ml and 1215.2 μg/ml, respectively.
Conclusion: The results of the present study indicate the potential anti-leishmanial effects of the ethanolic extract of N. koelzii on the promastigotes of Leishmania tropica. Induction of apoptosis may also be one of the most significant cellular mechanisms underlying the anti-parasitic effects of this extract. Additionally, the findings demonstrate that this extract exhibits low cytotoxicity towards normal human cells. However, further studies are needed to investigate the exact cellular mechanisms.
Type of Study: Research |
Subject:
انگل شناسی
Received: 2024/09/28 | Accepted: 2024/10/28 | Published: 2024/10/31
Received: 2024/09/28 | Accepted: 2024/10/28 | Published: 2024/10/31
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