Volume 27, Issue 4 (1-2026)
yafte 2026, 27(4): 27-40 |
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Lashani M, Ahmadi R, Javid A, Rezaei Zarchi S. Evaluation of the Cytotoxic Effects of Silver Chloride Nanoparticles Synthesized by a Green Method and 5-Fluorouracil on Breast Cancer Cells (MCF7). yafte 2026; 27 (4) :27-40
URL: http://yafte.lums.ac.ir/article-1-3802-en.html
URL: http://yafte.lums.ac.ir/article-1-3802-en.html
Department of Biology, Faculty of Engineering and Sciences, Yazd University of Sciences and Arts, Yazd, Iran & Department of Biology, Faculty of Engineering and Sciences, Yazd University of Sciences and Arts, Yazd, Iran
Abstract: (72 Views)
Background: Silver nanoparticles have recently gained significant interest in cancer therapy. Meanwhile, the chemotherapeutic drug 5-fluorouracil (5FU), despite its efficacy, faces limitations such as drug resistance. This study aims to investigate and compare the cytotoxic effects of green-synthesized silver chloride nanoparticles and 5FU on MCF7 breast cancer cells.
Materials and Methods: Silver chloride nanoparticles were synthesized using Onopordum acanthium extract through a green synthesis method and characterized to confirm their properties. MCF7 cells were cultured under standard conditions and divided into the following groups: control (untreated), treated with different concentrations of AgCl nanoparticles, 5FU, or both. After 24 hours of treatment, cell viability was assessed using the MTT assay. Data were analyzed using one-way ANOVA.
Results: SEM, EDS, and XRD analyses confirmed the nanoscale nature of the synthesized AgCl particles. Treatment of MCF7 cells with AgCl concentrations above 6.25 µg/mL, 5FU above 15.62 µg/mL, and their combination resulted in a significant decrease in cell viability (P<0.05, P<0.01, and P<0.001, respectively). The IC₅₀ values for 5FU, AgCl, and the AgCl+5FU combination were estimated at 3.98, 13.2, and 0.708 µg/mL, respectively.
Conclusion: Green-synthesized silver chloride nanoparticles, particularly in combination with 5FU, exhibit strong cytotoxic potential against MCF7 breast cancer cells. These results indicate a promising route for developing novel, targeted therapeutic strategies for breast cancer treatment.
Materials and Methods: Silver chloride nanoparticles were synthesized using Onopordum acanthium extract through a green synthesis method and characterized to confirm their properties. MCF7 cells were cultured under standard conditions and divided into the following groups: control (untreated), treated with different concentrations of AgCl nanoparticles, 5FU, or both. After 24 hours of treatment, cell viability was assessed using the MTT assay. Data were analyzed using one-way ANOVA.
Results: SEM, EDS, and XRD analyses confirmed the nanoscale nature of the synthesized AgCl particles. Treatment of MCF7 cells with AgCl concentrations above 6.25 µg/mL, 5FU above 15.62 µg/mL, and their combination resulted in a significant decrease in cell viability (P<0.05, P<0.01, and P<0.001, respectively). The IC₅₀ values for 5FU, AgCl, and the AgCl+5FU combination were estimated at 3.98, 13.2, and 0.708 µg/mL, respectively.
Conclusion: Green-synthesized silver chloride nanoparticles, particularly in combination with 5FU, exhibit strong cytotoxic potential against MCF7 breast cancer cells. These results indicate a promising route for developing novel, targeted therapeutic strategies for breast cancer treatment.
Keywords: 5-Fluorouracil (5FU), Cytotoxicity, Green Synthesis, MCF7 Cancer Cells, Silver Chloride Nanoparticles
Type of Study: Original Research |
Subject:
فارماکولوژی
Received: 2025/01/11 | Accepted: 2025/12/18 | Published: 2026/01/30
Received: 2025/01/11 | Accepted: 2025/12/18 | Published: 2026/01/30
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